| pubmed-article:19955274 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19955274 | lifeskim:mentions | umls-concept:C0005768 | lld:lifeskim |
| pubmed-article:19955274 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:19955274 | lifeskim:mentions | umls-concept:C1295932 | lld:lifeskim |
| pubmed-article:19955274 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:19955274 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:19955274 | lifeskim:mentions | umls-concept:C0596495 | lld:lifeskim |
| pubmed-article:19955274 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
| pubmed-article:19955274 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:19955274 | pubmed:dateCreated | 2010-2-1 | lld:pubmed |
| pubmed-article:19955274 | pubmed:abstractText | Rapid detection and identification of Ehrlichia species improves clinical outcome for patients suspected of ehrlichiosis. We describe an assay that employs multilocus PCR and electrospray ionization mass spectrometry (PCR/ESI-MS) to detect and identify Ehrlichia species directly from blood specimens. The results were compared to those of a colorimetric microtiter PCR enzyme immunoassay (PCR-EIA) used as a diagnostic assay. Among 213 whole-blood samples collected from patients who were clinically suspected of ehrlichiosis from 1 May to 1 August 2008 at Vanderbilt University Hospital, 40 were positive for an Ehrlichia species by PCR/ESI-MS, giving a positive rate of 18.8%. In comparison to the PCR-EIA, PCR/ESI-MS possessed a sensitivity, a specificity, and positive and negative predictive values of 95.0%, 98.8%, 95.0%, and 98.8%, respectively. The 38 specimens that were positive for Ehrlichia by both PCR/ESI-MS and the PCR-EIA were further characterized to the species level, with 100% agreement between the two assays. In addition, Rickettsia rickettsii was detected by PCR/ESI-MS from four specimens that were confirmed retrospectively by serology and PCR-EIA. In three specimens, the PCR/ESI-MS assay identified Pseudomonas aeruginosa, Neisseria meningitidis, and Staphylococcus aureus; these were confirmed by culture and/or clinical diagnosis as being clinically relevant. From specimen processing to result reporting, the PCR/ESI-MS assay can be completed within 6 h, providing another laboratory tool for the diagnosis of ehrlichiosis. Moreover, this system may provide rapid detection and identification of additional pathogens directly from blood specimens. | lld:pubmed |
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| pubmed-article:19955274 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19955274 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19955274 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19955274 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19955274 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:19955274 | pubmed:issn | 1098-660X | lld:pubmed |
| pubmed-article:19955274 | pubmed:author | pubmed-author:TangYi-WeiYW | lld:pubmed |
| pubmed-article:19955274 | pubmed:author | pubmed-author:LiHaijingH | lld:pubmed |
| pubmed-article:19955274 | pubmed:author | pubmed-author:SampathRangar... | lld:pubmed |
| pubmed-article:19955274 | pubmed:author | pubmed-author:BlynLawrence... | lld:pubmed |
| pubmed-article:19955274 | pubmed:author | pubmed-author:SefersSusan... | lld:pubmed |
| pubmed-article:19955274 | pubmed:author | pubmed-author:EckerDavid... | lld:pubmed |
| pubmed-article:19955274 | pubmed:author | pubmed-author:MengShufangS | lld:pubmed |
| pubmed-article:19955274 | pubmed:author | pubmed-author:StrattonCharl... | lld:pubmed |
| pubmed-article:19955274 | pubmed:author | pubmed-author:EshooMark WMW | lld:pubmed |
| pubmed-article:19955274 | pubmed:author | pubmed-author:CrowderChris... | lld:pubmed |
| pubmed-article:19955274 | pubmed:author | pubmed-author:MatthewsHeath... | lld:pubmed |
| pubmed-article:19955274 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19955274 | pubmed:volume | 48 | lld:pubmed |
| pubmed-article:19955274 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19955274 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19955274 | pubmed:pagination | 472-8 | lld:pubmed |
| pubmed-article:19955274 | pubmed:dateRevised | 2010-9-27 | lld:pubmed |
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| pubmed-article:19955274 | pubmed:meshHeading | pubmed-meshheading:19955274... | lld:pubmed |
| pubmed-article:19955274 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:19955274 | pubmed:articleTitle | Detection and identification of Ehrlichia species in blood by use of PCR and electrospray ionization mass spectrometry. | lld:pubmed |
| pubmed-article:19955274 | pubmed:affiliation | Ibis Biosciences, Carlsbad, California 92008, USA. | lld:pubmed |
| pubmed-article:19955274 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19955274 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:19955274 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:19955274 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
| pubmed-article:19955274 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19955274 | lld:pubmed |
