Linked Life Data

19950204

Source:http://linkedlifedata.com/resource/pubmed/id/19950204

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-1-26
pubmed:abstractText
This study addresses the role of bone morphogenetic protein-7 (BMP-7) in chondrogenic and osteogenic differentiation of human bone marrow multipotent mesenchymal stromal cells (BM MSCs) in vitro. BM MSCs were expanded and differentiated in the presence or absence of BMP-7 in monolayer and three-dimensional cultures. After 3 days of stimulation, BMP-7 significantly inhibited MSC growth in expansion cultures. When supplemented in commonly used induction media for 7-21 days, BMP-7 facilitated both chondrogenic and osteogenic differentiation of MSCs. This was evident by specific gene and protein expression analyses using real-time PCR, Western blot, histological, and immunohistochemical staining. BMP-7 supplementation appeared to enhance upregulation of lineage-specific markers, such as type II and type IX collagens (COL2A1, COL9A1) in chondrogenic and secreted phosphoprotein 1 (SPP1), osteocalcin (BGLAP), and osterix (SP7) in osteogenic differentiation. BMP-7 in the presence of TGF-beta3 induced superior chondrocytic proteoglycan accumulation, type II collagen, and SOX9 protein expression in alginate and pellet cultures compared to either factor alone. BMP-7 increased alkaline phosphatase activity and dose-dependently accelerated calcium mineralization of osteogenic differentiated MSCs. The potential of BMP-7 to promote adipogenesis of MSCs was restricted under osteogenic conditions, despite upregulation of adipocyte gene expression. These data suggest that BMP-7 is not a singular lineage determinant, rather it promotes both chondrogenic and osteogenic differentiation of MSCs by co-ordinating with initial lineage-specific signals to accelerate cell fate determination. BMP-7 may be a useful enhancer of in vitro differentiation of BM MSCs for cell-based tissue repair.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1097-4644
pubmed:author
pubmed:copyrightInfo
(c) 2009 Wiley-Liss, Inc.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
109
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
406-16
pubmed:meshHeading
pubmed-meshheading:19950204-Adipogenesis, pubmed-meshheading:19950204-Alginates, pubmed-meshheading:19950204-Alkaline Phosphatase, pubmed-meshheading:19950204-Antigens, Differentiation, pubmed-meshheading:19950204-Bone Marrow Cells, pubmed-meshheading:19950204-Bone Morphogenetic Protein 7, pubmed-meshheading:19950204-Calcification, Physiologic, pubmed-meshheading:19950204-Cell Culture Techniques, pubmed-meshheading:19950204-Cell Lineage, pubmed-meshheading:19950204-Cells, Cultured, pubmed-meshheading:19950204-Chondrogenesis, pubmed-meshheading:19950204-Glucuronic Acid, pubmed-meshheading:19950204-Hexuronic Acids, pubmed-meshheading:19950204-Humans, pubmed-meshheading:19950204-Mesenchymal Stem Cells, pubmed-meshheading:19950204-Multipotent Stem Cells, pubmed-meshheading:19950204-Osteogenesis, pubmed-meshheading:19950204-Proteoglycans, pubmed-meshheading:19950204-SOX9 Transcription Factor, pubmed-meshheading:19950204-Stromal Cells, pubmed-meshheading:19950204-Transforming Growth Factor beta3
pubmed:year
2010
pubmed:articleTitle
The role of BMP-7 in chondrogenic and osteogenic differentiation of human bone marrow multipotent mesenchymal stromal cells in vitro.
pubmed:affiliation
Orthopaedic Research Institute, Department of Orthopaedic Surgery, St. George Hospital, University of New South Wales, Sydney, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't