Linked Life Data

1993702

Source:http://linkedlifedata.com/resource/pubmed/id/1993702

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1991-3-20
pubmed:databankReference
pubmed:abstractText
Transcription of the chicken cardiac troponin T (cTNT) gene in myocardial cells requires upstream sequences not required for expression of this gene in embryonic skeletal muscle cells. Deletion analysis shows that the segment between nucleotides -247 and -201 (numbered relative to the transcription initiation site at +1) is capable of conferring cardiac specific expression to a "minimal" cTNT promoter. The cardiac element within this segment contains at least two essential subregions: one residing upstream of position -215, which bears no homologies to known transcription elements, and an A/T-rich segment residing between positions -215 and -201. Conserved M-CAT motifs within the cTNT minimal promoter which are required for activity in skeletal muscle cells are also required for activity in myocardial cells, suggesting an interaction between the upstream cardiac element and proximal promoter elements. Gel-shift experiments demonstrate interaction between the upstream portion of the cardiac element and factor(s) present in the nuclei of cardiac and non-cardiac tissues. Thus, additional cis and trans factors are required for transcription of the cTNT gene in myocardial cells which are not required for expression in skeletal muscle cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
266
pubmed:geneSymbol
cTNT
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3309-16
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Characterization of a promoter element required for transcription in myocardial cells.
pubmed:affiliation
Department of Anatomy, University of California, San Francisco 94143.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't