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pubmed-article:1988159pubmed:abstractTextThree thymic epithelial cell lines (TEC1C5, TEC1-4, and TEC2-3) were established from the thymus of newborn C57BL/6 mice. TEC1C5 was revealed to be an interleukin (IL)-1 producing cell line. TEC1-4 produced a cofactor to promote proliferation of double negative (CD4-8-) thymic lymphocytes by the presence of IL-1. Production of the same cofactor was also seen in TEC2-3, but only when it was cultured by the presence of indomethacin. The chemical analysis of the TEC1-4 culture supernatant by ion-exchange column and sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the factor was approximately 35 kDa in molecular weight. The present study revealed that a factor produced by TEC1-4 acted as a cofactor to promote the proliferation of immature T cells stimulated by IL-1 and various mitogens and was considered to be a new one in terms of molecular weight.lld:pubmed
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pubmed-article:1988159pubmed:authorpubmed-author:KasaiMMlld:pubmed
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pubmed-article:1988159pubmed:pagination377-90lld:pubmed
pubmed-article:1988159pubmed:dateRevised2003-11-14lld:pubmed
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pubmed-article:1988159pubmed:articleTitleA novel cofactor produced by a thymic epithelial cell line: promotion of proliferation of immature thymic lymphocytes by the presence of interleukin-1 and various mitogens.lld:pubmed
pubmed-article:1988159pubmed:affiliationDepartment of Pathology, Tokyo Metropolitan Institute of Gerontology, Japan.lld:pubmed
pubmed-article:1988159pubmed:publicationTypeJournal Articlelld:pubmed
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