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pubmed-article:19815608pubmed:abstractTextHuman embryonic stem cells (hESCs) require expression of transcription factor genes POU5F1 (POU class 5 homeobox 1), NANOG (Nanog homeobox), and SOX2 [SRY (sex determining region Y)-box 2] to maintain their capacity for self-renewal and pluripotency. Because of the heterogeneous nature of cell populations, it is desirable to study the gene regulation in single cells. Large and potentially important fluctuations in a few cells cannot be detected at the population scale with microarrays or sequencing technologies. We used single-cell gene expression profiling to study cell heterogeneity in hESCs.lld:pubmed
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pubmed-article:19815608pubmed:year2009lld:pubmed
pubmed-article:19815608pubmed:articleTitleQuantitative transcription factor analysis of undifferentiated single human embryonic stem cells.lld:pubmed
pubmed-article:19815608pubmed:affiliationLundberg Laboratory for Cancer, Department of Pathology, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden. anders.stahlberg@tataa.comlld:pubmed
pubmed-article:19815608pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19815608pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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