Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-1-26
pubmed:abstractText
Monitoring pathology/regeneration in experimental models of de-/remyelination requires an accurate measure not only of functional changes but also of the amount of myelin. We tested whether X-ray diffraction (XRD), which measures periodicity in unfixed myelin, can assess the structural integrity of myelin in fixed tissue. From laboratories involved in spinal cord injury research and in studying the aging primate brain, we solicited "blind" samples and used an electronic detector to record rapidly the diffraction patterns (30 min each pattern) from them. We assessed myelin integrity by measuring its periodicity and relative amount. Fixation of tissue itself introduced +/-10% variation in periodicity and +/-40% variation in relative amount of myelin. For samples having the most native-like periods, the relative amounts of myelin detected allowed distinctions to be made between normal and demyelinating segments, between motor and sensory tracts within the spinal cord, and between aged and young primate CNS. Different periodicities also allowed distinctions to be made between samples from spinal cord and nerve roots and between well-fixed and poorly fixed samples. Our findings suggest that, in addition to evaluating the effectiveness of different fixatives, XRD could also be used as a robust and rapid technique for quantitating the relative amount of myelin among spinal cords and other CNS tissue samples from experimental models of de- and remyelination.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-11135234, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-12151541, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-12151546, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-122265, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-13892941, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-15846782, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-16049170, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-16254492, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-16449196, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-16495463, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-16629621, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-17951861, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-18812651, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-1944805, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-2752082, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-2809635, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-479295, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-5283387, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-5769639, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-6163867, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-618435, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-7251668, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-8594199, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-9165491, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-953750, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-971429, http://linkedlifedata.com/resource/pubmed/commentcorrection/19795370-9932868
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1097-4547
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
712-21
pubmed:dateRevised
2011-7-25
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Rapid assessment of internodal myelin integrity in central nervous system tissue.
pubmed:affiliation
Biology Department, Boston College, Chestnut Hill, Massachusetts, USA. kirschnd@bc.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural