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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4974
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pubmed:dateCreated |
1990-10-17
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pubmed:databankReference |
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pubmed:abstractText |
A complementary DNA clone (designated GAT-1) encoding a transporter for the neurotransmitter gamma-aminobutyric acid (GABA) has been isolated from rat brain, and its functional properties have been examined in Xenopus oocytes. Oocytes injected with GAT-1 synthetic messenger RNA accumulated [3H]GABA to levels above control values. The transporter encoded by GAT-1 has a high affinity for GABA, is sodium-and chloride-dependent, and is pharmacologically similar to neuronal GABA transporters. The GAT-1 protein shares antigenic determinants with a native rat brain GABA transporter. The nucleotide sequence of GAT-1 predicts a protein of 599 amino acids with a molecular weight of 67 kilodaltons. Hydropathy analysis of the deduced protein suggests multiple transmembrane regions, a feature shared by several cloned transporters; however, database searches indicate that GAT-1 is not homologous to any previously identified proteins. Therefore, GAT-1 appears to be a member of a previously uncharacterized family of transport molecules.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorine,
http://linkedlifedata.com/resource/pubmed/chemical/GABA Plasma Membrane Transport...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Organic Anion Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Poly A,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/gamma-Aminobutyric Acid
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
249
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1303-6
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1975955-Amino Acid Sequence,
pubmed-meshheading:1975955-Animals,
pubmed-meshheading:1975955-Base Sequence,
pubmed-meshheading:1975955-Brain,
pubmed-meshheading:1975955-Carrier Proteins,
pubmed-meshheading:1975955-Chlorine,
pubmed-meshheading:1975955-Cloning, Molecular,
pubmed-meshheading:1975955-GABA Plasma Membrane Transport Proteins,
pubmed-meshheading:1975955-Gene Expression,
pubmed-meshheading:1975955-Membrane Proteins,
pubmed-meshheading:1975955-Membrane Transport Proteins,
pubmed-meshheading:1975955-Microinjections,
pubmed-meshheading:1975955-Molecular Sequence Data,
pubmed-meshheading:1975955-Nerve Tissue Proteins,
pubmed-meshheading:1975955-Oocytes,
pubmed-meshheading:1975955-Organic Anion Transporters,
pubmed-meshheading:1975955-Poly A,
pubmed-meshheading:1975955-RNA, Messenger,
pubmed-meshheading:1975955-Rats,
pubmed-meshheading:1975955-Sodium,
pubmed-meshheading:1975955-Structure-Activity Relationship,
pubmed-meshheading:1975955-Xenopus,
pubmed-meshheading:1975955-gamma-Aminobutyric Acid
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pubmed:year |
1990
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pubmed:articleTitle |
Cloning and expression of a rat brain GABA transporter.
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pubmed:affiliation |
Division of Biology, California Institute of Technology, Pasadena 91125.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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