| pubmed-article:19684016 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19684016 | lifeskim:mentions | umls-concept:C1883036 | lld:lifeskim |
| pubmed-article:19684016 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:19684016 | lifeskim:mentions | umls-concept:C1538050 | lld:lifeskim |
| pubmed-article:19684016 | lifeskim:mentions | umls-concept:C0379528 | lld:lifeskim |
| pubmed-article:19684016 | pubmed:issue | 41 | lld:pubmed |
| pubmed-article:19684016 | pubmed:dateCreated | 2009-10-5 | lld:pubmed |
| pubmed-article:19684016 | pubmed:abstractText | Gamma-secretase is a membrane protein complex that catalyzes intramembrane proteolysis of a variety of substrates including the amyloid beta precursor protein of Alzheimer disease. Nicastrin (NCT), a single-pass membrane glycoprotein that harbors a large extracellular domain, is an essential component of the gamma-secretase complex. Here we report that overexpression of a single chain variable fragment (scFv) against NCT as an intrabody suppressed the gamma-secretase activity. Biochemical analyses revealed that the scFv disrupted the proper folding and the appropriate glycosyl maturation of the endogenous NCT, which are required for the stability of the gamma-secretase complex and the intrinsic proteolytic activity, respectively, implicating the dual role of NCT in the gamma-secretase complex. Our results also highlight the importance of the calnexin cycle in the functional maturation of the gamma-secretase complex. The engineered intrabodies may serve as rationally designed, molecular targeting tools for the discovery of novel actions of the membrane proteins. | lld:pubmed |
| pubmed-article:19684016 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19684016 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19684016 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19684016 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19684016 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19684016 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19684016 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19684016 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19684016 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19684016 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19684016 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19684016 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19684016 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:19684016 | pubmed:issn | 1083-351X | lld:pubmed |
| pubmed-article:19684016 | pubmed:author | pubmed-author:RossL HLH | lld:pubmed |
| pubmed-article:19684016 | pubmed:author | pubmed-author:KodamaTatsuhi... | lld:pubmed |
| pubmed-article:19684016 | pubmed:author | pubmed-author:IwatsuboTakes... | lld:pubmed |
| pubmed-article:19684016 | pubmed:author | pubmed-author:TomitaTaisuke... | lld:pubmed |
| pubmed-article:19684016 | pubmed:author | pubmed-author:HamakuboTakao... | lld:pubmed |
| pubmed-article:19684016 | pubmed:author | pubmed-author:WongPhilip... | lld:pubmed |
| pubmed-article:19684016 | pubmed:author | pubmed-author:IwanariHiroko... | lld:pubmed |
| pubmed-article:19684016 | pubmed:author | pubmed-author:HayashiIkuoI | lld:pubmed |
| pubmed-article:19684016 | pubmed:author | pubmed-author:UranoYasuomiY | lld:pubmed |
| pubmed-article:19684016 | pubmed:author | pubmed-author:IsooNorikoN | lld:pubmed |
| pubmed-article:19684016 | pubmed:author | pubmed-author:TakatoriShoS | lld:pubmed |
| pubmed-article:19684016 | pubmed:author | pubmed-author:OsawaSatokoS | lld:pubmed |
| pubmed-article:19684016 | pubmed:author | pubmed-author:FukudaMaiko... | lld:pubmed |
| pubmed-article:19684016 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19684016 | pubmed:day | 9 | lld:pubmed |
| pubmed-article:19684016 | pubmed:volume | 284 | lld:pubmed |
| pubmed-article:19684016 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19684016 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19684016 | pubmed:pagination | 27838-47 | lld:pubmed |
| pubmed-article:19684016 | pubmed:dateRevised | 2010-10-12 | lld:pubmed |
| pubmed-article:19684016 | pubmed:meshHeading | pubmed-meshheading:19684016... | lld:pubmed |
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| pubmed-article:19684016 | pubmed:meshHeading | pubmed-meshheading:19684016... | lld:pubmed |
| pubmed-article:19684016 | pubmed:meshHeading | pubmed-meshheading:19684016... | lld:pubmed |
| pubmed-article:19684016 | pubmed:meshHeading | pubmed-meshheading:19684016... | lld:pubmed |
| pubmed-article:19684016 | pubmed:meshHeading | pubmed-meshheading:19684016... | lld:pubmed |
| pubmed-article:19684016 | pubmed:meshHeading | pubmed-meshheading:19684016... | lld:pubmed |
| pubmed-article:19684016 | pubmed:meshHeading | pubmed-meshheading:19684016... | lld:pubmed |
| pubmed-article:19684016 | pubmed:meshHeading | pubmed-meshheading:19684016... | lld:pubmed |
| pubmed-article:19684016 | pubmed:meshHeading | pubmed-meshheading:19684016... | lld:pubmed |
| pubmed-article:19684016 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19684016 | pubmed:articleTitle | Single chain variable fragment against nicastrin inhibits the gamma-secretase activity. | lld:pubmed |
| pubmed-article:19684016 | pubmed:affiliation | Department of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. | lld:pubmed |
| pubmed-article:19684016 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19684016 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:23385 | entrezgene:pubmed | pubmed-article:19684016 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:19684016 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19684016 | lld:pubmed |
