Linked Life Data

19657746

Source:http://linkedlifedata.com/resource/pubmed/id/19657746

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2009-9-4
pubmed:abstractText
Autophagy is a highly conserved pathway for the degradation and recycling of long-lived proteins and cytoplasmic organelles. Similar to apoptosis, autophagy is a critical regulatory mechanism for determining cellular fate and various pathophysiological conditions in metazoans. So far, the systematic analysis of the expression patterns and transcriptional regulation of autophagy-related (ATG) genes has remained incompletely defined. In this study, we used RT-PCR to analyze the expression patterns of 26 human ATG genes simultaneously using cDNA derived from different adult and fetal tissues. As a result, we observed a characteristic ubiquitous expression pattern for all the genes except for ATG2A, ATG9B, and WIPI2. In particular, ATG2A was the only upregulated gene in the etoposide-induced apoptosis of HeLa cells. ATG2A mRNA was also upregulated by doxorubicin. Furthermore, we demonstrated that 13 out of 23 human ATG gene promoters were regulated by the transcription factor E2F1 in HeLa cells, indicating that these constructs could be useful for examining how the autophagy pathway is involved in other cellular phenomena, such as apoptosis evoked by various stimuli. Taken together, these results suggest that autophagy might be regulated at both the transcriptional level and the post-translational level.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1573-675X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1165-75
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Comprehensive analysis of expression pattern and promoter regulation of human autophagy-related genes.
pubmed:affiliation
Department of Life Sciences, Faculty of Agriculture, Meiji University, Tama-ku, Kawasaki, Kanagawa, 214-8571, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't