In the present study changes of components of Wnt signaling pathway--axin (AXIN1) and beta-catenin (CTNNB1) in a sample of 72 neuroepithelial brain tumors were investigated. AXIN-1 gene was tested by PCR/loss of heterozygosity (LOH). Immunostaining and image analysis revealed the quantity and localization of relevant proteins. Polymorphic marker for AXIN-1, showed LOH in 11.1% of tumors. LOH was distributed to 6.3% of glioblastomas, one was found in neuroepithelial dysembrioplastic tumor and one in medulloblastoma. Down regulation of axin expression and up regulation of beta-catenin were detected in the analyzed tumors. Axin was observed in the cytoplasm in 68.8% of samples, in 28.1% in both the cytoplasm and nucleus and 3.1% had no expression. Beta-catenin was observed mainly in the nucleus and cytoplasm (59.4%). Expression in 34.4% of samples was in the cytoplasm and 6.3% showed no expression. Comparison of mean values of relative increase of axin and beta-catenin showed that they are significantly reversely proportional (P = 0.014). Relative quantity of beta-catenin in patients with gross deletion of AXIN1 was significantly higher in comparison to patients without LOH (P = 0.040). Our results demonstrate that changes of key components of the Wnt signaling play a role in neuroepithelial brain tumors.
http://linkedlifedata.com/resource/pubmed/chemical/AXIN1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Axin Protein, http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins, http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin
pubmed-meshheading:19633924-Axin Protein, pubmed-meshheading:19633924-Brain Neoplasms, pubmed-meshheading:19633924-Gene Expression, pubmed-meshheading:19633924-Gene Expression Profiling, pubmed-meshheading:19633924-Humans, pubmed-meshheading:19633924-Image Interpretation, Computer-Assisted, pubmed-meshheading:19633924-Immunohistochemistry, pubmed-meshheading:19633924-Loss of Heterozygosity, pubmed-meshheading:19633924-Neoplasms, Neuroepithelial, pubmed-meshheading:19633924-Polymerase Chain Reaction, pubmed-meshheading:19633924-Repressor Proteins, pubmed-meshheading:19633924-Signal Transduction, pubmed-meshheading:19633924-Wnt Proteins, pubmed-meshheading:19633924-beta Catenin
Changes of AXIN-1 and beta-catenin in neuroepithelial brain tumors.
Laboratory of Neurooncology, Croatian Institute for Brain Research, School of Medicine University of Zagreb, Salata 12, 10000, Zagreb, Croatia.
Journal Article, Research Support, Non-U.S. Gov't