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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2010-3-3
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pubmed:abstractText |
MicroRNA-based short hairpin RNAs (shRNAs) are natural inducers of RNA interference and have been increasingly used in shRNA expression strategies. In the present study, we compared the efficiencies of exogenous green fluorescence protein (GFP) and endogenous glyceraldehyde-3-phosphate dehydrogenase (GAPDH) knockdown and red fluorescent protein (RFP) indicator expression mediated by three differently designed plasmids. RFP was introduced either at the 5' end, at the 3' end of the human mir155-based target gene (TG) (e.g., GFP or GAPDH) shRNA expression cassette (EC), or at the 3' end of the chimeric intron-containing TG shRNA EC. Comparisons with the control vector showed an obvious reduction of GFP or GAPDH expression with the various shRNA expression plasmids (P < 0.05). When RFP was located at the 5' end or at the 3' end of the TG shRNA EC, RFP expression was low; whereas when RFP was connected with the chimeric intron-containing TG shRNA EC, RFP expression was high. Taken together, this study demonstrated an efficient plasmid design for both TG silencing induced by microRNA-based shRNA and indicator gene expression in vitro.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glyceraldehyde-3-Phosphate...,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MIRN155 microRNA, human,
http://linkedlifedata.com/resource/pubmed/chemical/MicroRNAs,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/red fluorescent protein
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1573-4978
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pubmed:author |
pubmed-author:DayE PEP,
pubmed-author:FreeE EEE,
pubmed-author:FuY HYH,
pubmed-author:KAOT MTM,
pubmed-author:KuangS JSJ,
pubmed-author:LiX HXH,
pubmed-author:PERM IMI,
pubmed-author:ShaoZ YZY,
pubmed-author:TaxH RHR,
pubmed-author:YuX YXY,
pubmed-author:ZhouZ LZL,
pubmed-author:ZhuJ NJN
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pubmed:issnType |
Electronic
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1831-9
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pubmed:meshHeading |
pubmed-meshheading:19603286-Base Sequence,
pubmed-meshheading:19603286-Cell Line,
pubmed-meshheading:19603286-Gene Expression Regulation,
pubmed-meshheading:19603286-Gene Silencing,
pubmed-meshheading:19603286-Genetic Techniques,
pubmed-meshheading:19603286-Glyceraldehyde-3-Phosphate Dehydrogenases,
pubmed-meshheading:19603286-Green Fluorescent Proteins,
pubmed-meshheading:19603286-Humans,
pubmed-meshheading:19603286-Luciferases,
pubmed-meshheading:19603286-Luminescent Proteins,
pubmed-meshheading:19603286-MicroRNAs,
pubmed-meshheading:19603286-Molecular Sequence Data,
pubmed-meshheading:19603286-Nucleic Acid Conformation,
pubmed-meshheading:19603286-Plasmids,
pubmed-meshheading:19603286-RNA, Messenger,
pubmed-meshheading:19603286-RNA, Small Interfering,
pubmed-meshheading:19603286-Transfection
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pubmed:year |
2010
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pubmed:articleTitle |
Comparison of approaches for efficient gene silencing induced by microRNA-based short hairpin RNA and indicator gene expression.
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pubmed:affiliation |
Research Center of Medical Sciences, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, China.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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