Source:http://linkedlifedata.com/resource/pubmed/id/19582038
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
14
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pubmed:dateCreated |
2009-7-7
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pubmed:abstractText |
We demonstrate two complementary types of microscopy using an identical setup for single-pulse coherent anti-Stokes Raman scattering (CARS) imaging, which employs an ultrabroadband laser pulse with a spectral bandwidth of 4800 cm(-1) and enables the suppression of nonresonant CARS signals. One is a novel type of microscopy that uses spectral phase modulation for the selective excitation of a single Raman mode. The selective excitation is achieved by the modulated pulse focusing its difference-frequency spectrum into a narrow spectral region. Another type is Fourier-transform CARS (FT-CARS) microspectroscopy based on the measurement of the CARS spectrum obtained from the Fourier-transform of the interferometric autocorrelation (IAC) signal. Vibrational spectral imaging of chemical and biological samples is demonstrated using the two types of microscopy.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1094-4087
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
6
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11259-66
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pubmed:meshHeading |
pubmed-meshheading:19582038-Algorithms,
pubmed-meshheading:19582038-Equipment Design,
pubmed-meshheading:19582038-Fourier Analysis,
pubmed-meshheading:19582038-HeLa Cells,
pubmed-meshheading:19582038-Humans,
pubmed-meshheading:19582038-Interferometry,
pubmed-meshheading:19582038-Lasers,
pubmed-meshheading:19582038-Mitochondria,
pubmed-meshheading:19582038-Optics and Photonics,
pubmed-meshheading:19582038-Spectrum Analysis, Raman,
pubmed-meshheading:19582038-Time Factors
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pubmed:year |
2009
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pubmed:articleTitle |
Single-pulse coherent anti-Stokes Raman scattering microscopy employing an octave spanning pulse.
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pubmed:affiliation |
Keisuke Isobe Laser Technology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. kisobe@riken.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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