Source:http://linkedlifedata.com/resource/pubmed/id/19444697
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2010-7-2
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| pubmed:abstractText |
Neurovirulence is one of the pathological complications associated with vaccinia virus (VV) infection/vaccination. Although the viral N1L protein has been identified as the neurovirulence factor, none of the host N1L-interacting factors have been identified so far. In the present study, we identified N1L-interacting proteins by screening a human brain cDNA expression library with N1L as a bait protein in a yeast two-hybrid analysis. The analysis revealed that N1L interacts with human brain-originated cellular basement membrane-associated chondroitin sulfate proteoglycan (bamacan). The N1L-binding domain of bamacan was mapped to its C-terminal 227 amino acids. The N1L-bamacan interaction was further confirmed in both VV-infected and N1L-transfected mammalian cells. Following the confirmation of the protein interactions by coimmunoprecipitation experiments, confocal microscopic analysis revealed that N1L colocalizes with bamacan both in VV-infected B-SC-1 cells as well as in mice neuronal tissue. Furthermore, a human neural cell line, which expresses bamacan to moderately elevated levels relative to a non-neural cell line, supported enhanced viral growth. Overall, these studies clearly suggest that bamacan interacts with the VV-N1L and such interactions seem to play a positive role in promoting the viral growth and perhaps contribute to the virulence of VV in neural cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chondroitin Sulfate Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/Cspg6 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/N1L protein, Vaccinia virus,
http://linkedlifedata.com/resource/pubmed/chemical/SMC3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1538-2443
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
15
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
229-37
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| pubmed:dateRevised |
2011-3-29
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| pubmed:meshHeading |
pubmed-meshheading:19444697-Animals,
pubmed-meshheading:19444697-Animals, Suckling,
pubmed-meshheading:19444697-Brain,
pubmed-meshheading:19444697-COS Cells,
pubmed-meshheading:19444697-Cell Cycle Proteins,
pubmed-meshheading:19444697-Cell Line, Tumor,
pubmed-meshheading:19444697-Cercopithecus aethiops,
pubmed-meshheading:19444697-Chondroitin Sulfate Proteoglycans,
pubmed-meshheading:19444697-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:19444697-Host-Pathogen Interactions,
pubmed-meshheading:19444697-Humans,
pubmed-meshheading:19444697-Ligands,
pubmed-meshheading:19444697-Mice,
pubmed-meshheading:19444697-Protein Binding,
pubmed-meshheading:19444697-Vaccinia,
pubmed-meshheading:19444697-Vaccinia virus,
pubmed-meshheading:19444697-Vero Cells,
pubmed-meshheading:19444697-Viral Proteins
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| pubmed:year |
2009
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| pubmed:articleTitle |
The proteoglycan bamacan is a host cellular ligand of vaccinia virus neurovirulence factor N1L.
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| pubmed:affiliation |
Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA. krishna.ketha391534@fda.hhs.gov
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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