Linked Life Data

19337837

Source:http://linkedlifedata.com/resource/pubmed/id/19337837

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2009-5-5
pubmed:abstractText
Molecular imaging of tumor antisense gene techniques have been applied to the study of magnetic resonance (MR) gene imaging associated with malignant tumors. In this study, we designed, synthesized, and tested a novel molecular probe, in which the antisense oligodeoxynucleotide (ASODN) was labeled with superparamagnetic iron oxide (SPIO), and its efficiency was examined by in vitro MR imaging after SK-Br-3 mammary carcinoma cell lines (oncocytes) transfection. The SPIO-labeled ASODN probe was prepared through SPIO conjugated to ASODN using a chemical cross linking method. Its morphology and size were detected by atomic force microscope, size distribution were detected by laser granulometer, the conjugating rate and biological activity were determined by high performance liquid chromatography, and the stability was determined by polyacrylamide gel electrophoresis. After that, the probes were transfected into the SK-Br-3 oncocytes, cellular iron uptake was analyzed qualitatively at light and electron microscopy and was quantified at atomic absorption spectrometry, and the signal change of the transfected cells was observed and measured using MR imaging. The morphology of the SPIO-labeled ASODN probe was mostly spherical with well-distributed scattering, and the diameters were between 25 and 40 nm (95%) by atomic force microscope and laser granulometer, the conjugating rate of the probe was 99%. Moreover, this probe kept its activity under physiological conditions and could conjugate with antisense oligodeoxynucleotide. In addition, light microscopy revealed an intracellular uptake of iron oxides in the cytosol and electron microscopic studies revealed a lysosomal deposition of iron oxides in the transfected SK-Br-3 oncocytes by antisense probes, some of them gathered stacks, and the iron content of the group of transfected SK-Br-3 oncocytes by antisense probe is significantly higher (18.37 +/- 0.42 pg) than other contrast groups, the MR imaging showed that transfected SK-Br-3 oncocytes by antisense probe had the lowest signal of all. The SPIO-labeled ASODN probe shows unique features including well-distributed spherical morphology, high conjugating rate and loading efficiency, and the signal intensity of SPIO-labeled ASODN-transfected SK-Br-3 oncocytes is reduced in MR imaging. These results indicate that the SPIO-labeled ASODN probe is potentially useful as a MR targeting contrast enhancing agent to specifically diagnose tumors which had over-expression of the c-erbB2 oncogene at an early stage.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1521-6047
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1240-50
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:19337837-Antisense Elements (Genetics), pubmed-meshheading:19337837-Breast Neoplasms, pubmed-meshheading:19337837-Cell Line, Tumor, pubmed-meshheading:19337837-Contrast Media, pubmed-meshheading:19337837-Dextrans, pubmed-meshheading:19337837-Ferumoxytol, pubmed-meshheading:19337837-Humans, pubmed-meshheading:19337837-Image Enhancement, pubmed-meshheading:19337837-Iron, pubmed-meshheading:19337837-Magnetic Resonance Imaging, pubmed-meshheading:19337837-Magnetite Nanoparticles, pubmed-meshheading:19337837-Microscopy, Atomic Force, pubmed-meshheading:19337837-Molecular Structure, pubmed-meshheading:19337837-Nanoparticles, pubmed-meshheading:19337837-Oligonucleotide Probes, pubmed-meshheading:19337837-Oxides, pubmed-meshheading:19337837-Oxyphil Cells, pubmed-meshheading:19337837-Staining and Labeling
pubmed:year
2009
pubmed:articleTitle
Preparation and quality test of superparamagnetic iron oxide labeled antisense oligodeoxynucleotide probe: a preliminary study.
pubmed:affiliation
Department of Radiology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't