Linked Life Data

19276588

Source:http://linkedlifedata.com/resource/pubmed/id/19276588

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-3-11
pubmed:abstractText
In this paper, we describe systematic detailed considerations of the feasibility of using various metal nanoparticles for organic-matrix-free surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). In order to avoid the influence of organic molecules on the nanoparticles, stabilizer-free bare nanoparticles of Ag, Au, Cu and Pt were prepared by laser ablation. Although all metal nanoparticles absorbed N(2) laser light (337 nm) energy, the performance of desorption/ionization of a representative peptide, angiotensin I, strongly depended on the metal element. Citrate buffer was used as a proton source; it reduced the amount of alkali cation adducts present. Then, protonated molecules of analytes predominated in the mass spectra when Au and Pt nanoparticles were used. Pt nanoparticles showed the highest performance in SALDI-MS, owing to their smaller heat conductivity and higher melting temperature. The selective desorption of a cationic surfactant with longer alkyl chains and a peptide with methionine was also observed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1348-2246
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
339-46
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Detailed investigation on the possibility of nanoparticles of various metal elements for surface-assisted laser desorption/ionization mass spectrometry.
pubmed:affiliation
Department of Chemistry, School of Science, The University of Tokyo, Hongo, Bunkyo, Tokyo 113-0033, Japan. tetsu@chem.s.u-tokyo.ac.jp
pubmed:publicationType
Journal Article