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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2009-4-28
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pubmed:abstractText |
Acidic conditions and the presence of exogenous agmatine are required to achieve maximal expression of the agmatine deiminase system (AgDS) of Streptococcus mutans. Here we demonstrate that the transcriptional activator of the AgDS, AguR, is required for the responses to agmatine and to low pH. Linker scanning mutagenesis was used to create a panel of mutated aguR genes that were utilized to complement an aguR deletion mutant of S. mutans. The level of production of the mutant proteins was shown to be comparable to that of the wild-type AguR protein. Mutations in the predicted DNA binding domain of AguR eliminated activation of the agu operon. Insertions into the region connecting the DNA binding domain to the predicted extracellular and transmembrane domains were well tolerated. In contrast, a variety of mutants were isolated that had a diminished capacity to respond to low pH but retained the ability to activate AgDS gene expression in response to agmatine, and vice versa. Also, a number of mutants were unable to respond to either agmatine or low pH. AguD, which is a predicted agmatine-putrescine antiporter, was found to be a negative regulator of AgDS gene expression in the absence of exogenous agmatine but was not required for low-pH induction of the AgDS genes. This study reveals that the control of AgDS gene expression by both agmatine and low pH is coordinated through the AguR protein and begins to identify domains of the protein involved in sensing and signaling.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19270124-10217779,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1098-5336
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2629-37
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pubmed:dateRevised |
2010-9-23
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pubmed:meshHeading |
pubmed-meshheading:19270124-Agmatine,
pubmed-meshheading:19270124-Bacterial Proteins,
pubmed-meshheading:19270124-Binding Sites,
pubmed-meshheading:19270124-Gene Expression Regulation, Bacterial,
pubmed-meshheading:19270124-Hydrogen-Ion Concentration,
pubmed-meshheading:19270124-Hydrolases,
pubmed-meshheading:19270124-Mutagenesis, Insertional,
pubmed-meshheading:19270124-Protein Structure, Tertiary,
pubmed-meshheading:19270124-Streptococcus mutans,
pubmed-meshheading:19270124-Trans-Activators
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pubmed:year |
2009
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pubmed:articleTitle |
AguR is required for induction of the Streptococcus mutans agmatine deiminase system by low pH and agmatine.
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pubmed:affiliation |
Department of Oral Biology, University of Florida, P.O. Box 100424, Gainesville, FL 32610-0424, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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