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rdf:type |
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|
lifeskim:mentions |
|
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pubmed:issue |
4
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pubmed:dateCreated |
2009-3-23
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pubmed:abstractText |
Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria.
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1879-0070
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pubmed:author |
pubmed-author:AbbottSharonS,
pubmed-author:BaldwinCarson DCD,
pubmed-author:BlynLarry BLB,
pubmed-author:DraderJared JJJ,
pubmed-author:EshooMark WMW,
pubmed-author:HallThomas ATA,
pubmed-author:HarpinVanessaV,
pubmed-author:HofstadlerSteve ASA,
pubmed-author:HoweGerald BGB,
pubmed-author:JandaJ MichaelJM,
pubmed-author:MatthewsHeatherH,
pubmed-author:MooreDavidD,
pubmed-author:RudnickKarlK,
pubmed-author:SampathRangaR,
pubmed-author:StudarusKarenK,
pubmed-author:WhitehouseChris ACA
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pubmed:issnType |
Electronic
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pubmed:volume |
63
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pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
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pubmed:pagination |
403-8
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pubmed:meshHeading |
pubmed-meshheading:19232863-Animals,
pubmed-meshheading:19232863-Bacteria,
pubmed-meshheading:19232863-Bacterial Infections,
pubmed-meshheading:19232863-DNA, Bacterial,
pubmed-meshheading:19232863-Humans,
pubmed-meshheading:19232863-Polymerase Chain Reaction,
pubmed-meshheading:19232863-Sensitivity and Specificity,
pubmed-meshheading:19232863-Spectrometry, Mass, Electrospray Ionization
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pubmed:year |
2009
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pubmed:articleTitle |
Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates.
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pubmed:affiliation |
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
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pubmed:publicationType |
Journal Article,
Evaluation Studies
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