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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 2
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pubmed:dateCreated |
2009-2-5
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pubmed:abstractText |
Cyanobacteriochromes form a recently defined superfamily of tetrapyrrole-based photoreceptors that are distantly related to conventional red/far-red photoreceptor phytochromes. Among these molecules, AnPixJ from Anabaena sp. PCC 7120 is a novel photoreceptor that shows reversible photoconversion between green-absorbing and red-absorbing forms, which is in contrast to the properties of conventional phytochromes. In order to better understand the structural basis of this unique photoconversion mechanism, the chromophore-binding domain of AnPixJ (AnPixJ-GAF2) was heterologously overproduced and purified, and crystallization of both forms was attempted. Blue crystals of the red-absorbing form of AnPixJ-GAF2 were successfully obtained; they belonged to space group P4(3)2(1)2 and contained one monomer per asymmetric unit. Diffraction data were collected to a resolution of 1.8 A using synchrotron-radiation beamline BL-5A at the Photon Factory.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-11557134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-11858227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-12167331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-12186972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-15170479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-15299374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-15653792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-16292304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-16458890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-16533061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-16669784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-16760311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-16887842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-17322301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-17640891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-17715149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-18571200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-18621684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-18846279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-18846291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19194010-9433123
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1744-3091
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
159-62
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pubmed:dateRevised |
2011-7-22
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pubmed:meshHeading |
pubmed-meshheading:19194010-Anabaena,
pubmed-meshheading:19194010-Bacterial Proteins,
pubmed-meshheading:19194010-Crystallization,
pubmed-meshheading:19194010-Crystallography, X-Ray,
pubmed-meshheading:19194010-Photoreceptors, Microbial,
pubmed-meshheading:19194010-Phycobilins,
pubmed-meshheading:19194010-Phycocyanin,
pubmed-meshheading:19194010-Phytochrome,
pubmed-meshheading:19194010-Protein Binding,
pubmed-meshheading:19194010-Protein Structure, Tertiary
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pubmed:year |
2009
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pubmed:articleTitle |
Crystallization and preliminary X-ray studies of the chromophore-binding domain of cyanobacteriochrome AnPixJ from Anabaena sp. PCC 7120.
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pubmed:affiliation |
Department of Life Sciences, Graduate School of Art and Sciences, University of Tokyo, Komaba, Meguro, Tokyo 153-8902, Japan. narikawa@bio.c.u-tokyo.ac.jp
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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