Linked Life Data

19149969

Source:http://linkedlifedata.com/resource/pubmed/id/19149969

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-1-19
pubmed:abstractText
Platelets form heterotypic complexes with circulating monocytes, inducing the expression of the procoagulant Tissue Factor (TF) that leads to thrombin generation. We investigated the potential preventive effect of melagatran, a direct anti-thrombin drug, on TF expression by platelet/monocyte heterotypic complexes (PMHC) from healthy human donors. Flow cytometry and western blot analysis were performed to characterize surface and total TF protein expression in PMHC in venous blood samples drawn in the presence of heparin or heparin and melagatran (4 microM). Addition of melagatran significantly lowered the percentage of TF positive PMHC (2.6+/-0.3 vs. 5.9+/-0.7 %, p<0.01). This was not due to a melagatran-induced decrease in activation of the platelets associated with monocytes in PMHC. Indeed, melagatran effect on TF expression was accompanied by an increase in cell surface P-selectin expression in PMHC (95.6+/-1.9 vs. 48+/-18 %, p<0.001), suggesting that platelet were actually more activated in PMHC from the melagatran-treated samples. Western blot analysis of PBMC extracts suggested that melagatran specifically targeted a (54kD) form of TF in monocytes. Although further investigation is warranted, these data suggest that melagatran decreases TF expression in PMHC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1165-158X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
54 Suppl
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
OL1077-82
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Melagatran prevents tissue factor expression in human platelet-monocyte heterotypic complexes.
pubmed:affiliation
Inserm U927, Poitiers, F-86021, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't