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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2008-11-25
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pubmed:abstractText |
Residual monomers in resin-based biomaterials cause cytotoxicity. We previously showed that methyl methacrylate (MMA) induced mRNA expression of the glutathione S-transferase alpha 1 gene (Gsta1) located downstream of the cis-acting anti-oxidant responsive element (ARE). Herein, we tested the hypothesis that MMA activated the Gsta1 promoter through the ARE. HepG2 cells were transfected with a luciferase reporter vector containing the ARE and the Gsta1 promoter (-990 to +46 bp) and cultured for 12 hrs with MMA (initial concentration, 10 mM). Analysis of the expressed luciferase activity indicated that MMA activated the promoter 2.6-fold. MMA (from 1 to 30 mM) dose-dependently increased the promoter activity, which reached a plateau between 6 and 12 hrs. In HepG2 cells transfected with a reporter vector containing 2 AREs and a TATA-like promoter, 10 mM MMA increased the reporter expression 2.8-fold. These results suggest that MMA increases Gsta1 transcription through ARE-mediated promoter activation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
D
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-tert-butylhydroquinone,
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Dental Materials,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroquinones,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Methylmethacrylate,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/glutathione S-transferase alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1544-0591
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pubmed:author |
pubmed-author:HattoriNN,
pubmed-author:HayashiTT,
pubmed-author:IshikawaAA,
pubmed-author:ItoMM,
pubmed-author:JinnoSS,
pubmed-author:KanamoriTT,
pubmed-author:KawaiTT,
pubmed-author:KondoCC,
pubmed-author:NoguchiTT,
pubmed-author:OkeyaHH,
pubmed-author:SuzukiTT
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pubmed:issnType |
Electronic
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1117-21
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pubmed:meshHeading |
pubmed-meshheading:19029078-5' Flanking Region,
pubmed-meshheading:19029078-Animals,
pubmed-meshheading:19029078-Antioxidants,
pubmed-meshheading:19029078-Cell Count,
pubmed-meshheading:19029078-Cell Line, Tumor,
pubmed-meshheading:19029078-Cell Survival,
pubmed-meshheading:19029078-Cells, Cultured,
pubmed-meshheading:19029078-Dental Materials,
pubmed-meshheading:19029078-Dose-Response Relationship, Drug,
pubmed-meshheading:19029078-Enzyme Inhibitors,
pubmed-meshheading:19029078-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:19029078-Genes, Reporter,
pubmed-meshheading:19029078-Genetic Vectors,
pubmed-meshheading:19029078-Glutathione Transferase,
pubmed-meshheading:19029078-Humans,
pubmed-meshheading:19029078-Hydroquinones,
pubmed-meshheading:19029078-Isoenzymes,
pubmed-meshheading:19029078-Luciferases,
pubmed-meshheading:19029078-Methylmethacrylate,
pubmed-meshheading:19029078-Mice,
pubmed-meshheading:19029078-Plasmids,
pubmed-meshheading:19029078-Promoter Regions, Genetic,
pubmed-meshheading:19029078-Reactive Oxygen Species,
pubmed-meshheading:19029078-Response Elements,
pubmed-meshheading:19029078-TATA Box,
pubmed-meshheading:19029078-Tandem Repeat Sequences,
pubmed-meshheading:19029078-Time Factors,
pubmed-meshheading:19029078-Transcriptional Activation,
pubmed-meshheading:19029078-Transfection
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pubmed:year |
2008
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pubmed:articleTitle |
Methyl methacrylate activates the Gsta1 promoter.
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pubmed:affiliation |
Department of Periodontology, School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan. srp-psho@dpc.agu.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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