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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1991-10-4
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pubmed:abstractText |
V pre-B and lambda-like genes are selectively expressed in human pre-B cells and encode polypeptide chains that associate in a mu-pseudolight chain complex that may regulate some crucial steps of early B-cell differentiation. We have followed by polymerase chain reaction and Northern blot analysis the expression of these "pre-B-specific" genes in correlation with the status (rearranged v germline) of Ig gene loci (H, kappa, lambda) in a panel of 32 leukemias pertaining mostly to the B lineage and including a number of ambiguously characterized samples. All cells that had rearranged the H locus only expressed V pre-B and lambda-like transcripts, in agreement with a pre-B status. In this group, some biphenotypic leukemias (mostly My/B) might, in fact, be already engaged in the B lineage. Rearrangement of V kappa or V lambda loci correlated with the disappearance of the pre-B gene products. In a pre-B acute lymphoblastic leukemia cell line that was induced to mature to the B-cell stage in culture upon kappa gene rearrangement, the mu-pseudolight chain complex was actually replaced by the classical mu-kappa molecule. Finally, V pre-B and lambda-like genes were found expressed in two leukemic cells that had retained all Ig loci in germline configuration. This finding raises the possibility of having an early pro-B progenitor in which V pre-B and lambda-like products associate with a H chain surrogate in a complex that would trigger an early event of B-cell differentiation such as the H locus rearrangements.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-4971
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
78
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pubmed:geneSymbol |
&lgr;,
V Pre-B
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1516-25
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1884018-Antigens, CD,
pubmed-meshheading:1884018-Base Sequence,
pubmed-meshheading:1884018-DNA, Neoplasm,
pubmed-meshheading:1884018-Gene Expression Regulation, Leukemic,
pubmed-meshheading:1884018-Gene Rearrangement, B-Lymphocyte,
pubmed-meshheading:1884018-Genes, Immunoglobulin,
pubmed-meshheading:1884018-Genetic Markers,
pubmed-meshheading:1884018-Humans,
pubmed-meshheading:1884018-Immunophenotyping,
pubmed-meshheading:1884018-Leukemia, B-Cell,
pubmed-meshheading:1884018-Molecular Sequence Data,
pubmed-meshheading:1884018-Polymerase Chain Reaction,
pubmed-meshheading:1884018-Protein Biosynthesis,
pubmed-meshheading:1884018-RNA, Neoplasm,
pubmed-meshheading:1884018-Transcription, Genetic
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pubmed:year |
1991
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pubmed:articleTitle |
Lambda-like and V pre-B genes expression: an early B-lineage marker of human leukemias.
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pubmed:affiliation |
Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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