Linked Life Data

18820671

Source:http://linkedlifedata.com/resource/pubmed/id/18820671

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2008-11-21
pubmed:abstractText
Diffuse C4d deposition in peritubular capillaries is a well-recognized marker of antibody-mediated rejection. The significance of staining patterns that are focal or affect non-peritubular capillary compartments is less well defined. Paired frozen section and paraffin-embedded tissue stains were performed in 52 kidney allograft biopsies, and correlated with clinicopathologic parameters. Diffuse peritubular capillary C4d deposits were more often seen in frozen sections (22/52, 43% frozen tissue vs 10/52, 19% paraffin-embedded tissue), whereas focal staining was observed more frequently within paraffin sections (13/52, 25% paraffin-embedded tissue vs 7/52, 14% frozen tissue). In biopsies taken from patients with a history of donor-specific antibodies, diffuse, focal and negative peritubular capillary C4d staining patterns were seen in 11/14 (79%), 1/14 (7%) and 2/14 (14%) of frozen biopsies vs 5/14 (36%), 6/14 (43%) and 3/14 (21%) of paraffin-embedded biopsies. Transplant glomerulopathy score in paraffin-embedded biopsies was higher in specimens with vs without glomerular basement membrane C4d staining (1.5+/-0.8 vs 1.0+/-0.6, P=0.03). Tubular basement membrane staining was present in 4% paraffin-embedded and 48% frozen specimens independent of tubular atrophy. Arteriolar hyalinosis score in paraffin-embedded specimens was higher in biopsies with vs those without arteriolar C4d deposits (1.3+/-0.9 vs 0.9+/-0.8, P=0.04). Arterial staining was unrelated to the degree of intimal thickening. In conclusion, peritubular capillary deposits correlate well with circulating donor-specific antibody. For paraffin-embedded tissue, combining the results of focal and diffuse staining allows a diagnostic sensitivity comparable to diffuse staining in frozen tissue. Finally, C4d deposits preferentially in lesions of chronic transplant glomerulopathy and arteriolar hyalinosis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1530-0285
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1490-8
pubmed:meshHeading
pubmed-meshheading:18820671-Adult, pubmed-meshheading:18820671-Aged, pubmed-meshheading:18820671-Aged, 80 and over, pubmed-meshheading:18820671-Capillaries, pubmed-meshheading:18820671-Complement C4b, pubmed-meshheading:18820671-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:18820671-Female, pubmed-meshheading:18820671-Frozen Sections, pubmed-meshheading:18820671-Glomerular Basement Membrane, pubmed-meshheading:18820671-Graft Rejection, pubmed-meshheading:18820671-Humans, pubmed-meshheading:18820671-Isoantibodies, pubmed-meshheading:18820671-Isoantigens, pubmed-meshheading:18820671-Kidney Transplantation, pubmed-meshheading:18820671-Kidney Tubules, pubmed-meshheading:18820671-Male, pubmed-meshheading:18820671-Middle Aged, pubmed-meshheading:18820671-Paraffin Embedding, pubmed-meshheading:18820671-Peptide Fragments, pubmed-meshheading:18820671-Sensitivity and Specificity, pubmed-meshheading:18820671-Transplantation, Homologous
pubmed:year
2008
pubmed:articleTitle
Clinical significance of the distribution of C4d deposits in different anatomic compartments of the allograft kidney.
pubmed:affiliation
Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural