|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0011306,
umls-concept:C0019721,
umls-concept:C0021079,
umls-concept:C0021080,
umls-concept:C0039198,
umls-concept:C0085358,
umls-concept:C0086418,
umls-concept:C0456387,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1546857,
umls-concept:C1704675,
umls-concept:C1706438,
umls-concept:C2698600
|
|
pubmed:issue |
11
|
|
pubmed:dateCreated |
2008-11-25
|
|
pubmed:abstractText |
We previously reported autoreactive CD8(+) regulatory T cells (Tregs) that were expanded and cloned from human peripheral blood by coculture with autologous dendritic cells (DC). Here we demonstrate that these CD8(+) Tregs require human leukocyte antigen (HLA)-class I restricted activation and then mediate cell-contact-dependent suppression of CD4(+) T cells. CD8(+) Tregs interacted with DC to suppress T-cell responses but DC were not irreversibly altered by this interaction because they could subsequently stimulate CD4(+) T cells normally. The ability of DC to form conjugates with CD4(+) T cells was reduced in the presence of CD8(+) Tregs. Suppression was blocked by Abs to CD80 and CTLA-4, implicating CTLA-4:CD80 interactions in the function of CD8(+) Tregs. CD8(+) Tregs rapidly express very high levels of surface CTLA-4 following activation compared with conventional T cells. Related to this, the expression of TRAT1 mRNA (T-cell receptor interacting molecule, or TRIM) was highly upregulated in microarray analysis of CD8(+) Tregs compared with conventional cytotoxic or nonregulatory CD8(+) T cells. TRIM acts to chaperone CTLA-4 transport to the cell surface; this function would be required to account for the phenotypic and functional properties of CD8(+) Tregs.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/CTLA-4 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/CTLA4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class I,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/TRIM protein, human
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0198-8859
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
69
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
687-95
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:18817831-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:18817831-Antibodies,
pubmed-meshheading:18817831-Antigens, CD,
pubmed-meshheading:18817831-Antigens, CD80,
pubmed-meshheading:18817831-CD4-Positive T-Lymphocytes,
pubmed-meshheading:18817831-CD8-Positive T-Lymphocytes,
pubmed-meshheading:18817831-CTLA-4 Antigen,
pubmed-meshheading:18817831-Cell Communication,
pubmed-meshheading:18817831-Dendritic Cells,
pubmed-meshheading:18817831-Gene Expression Profiling,
pubmed-meshheading:18817831-Histocompatibility Antigens Class I,
pubmed-meshheading:18817831-Humans,
pubmed-meshheading:18817831-Immune Tolerance,
pubmed-meshheading:18817831-Lymphocyte Activation,
pubmed-meshheading:18817831-Membrane Proteins,
pubmed-meshheading:18817831-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:18817831-RNA, Messenger,
pubmed-meshheading:18817831-Up-Regulation
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Human leukocyte antigen class I-restricted immunosuppression by human CD8+ regulatory T cells requires CTLA-4-mediated interaction with dendritic cells.
|
|
pubmed:affiliation |
Department of Rheumatology, University of Cambridge, Cambridge, UK.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
