Linked Life Data

18719336

Source:http://linkedlifedata.com/resource/pubmed/id/18719336

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-8-22
pubmed:abstractText
Kidney and prostate cancers are leading causes of death in the world, and accumulating evidence suggests that tumor suppressor gene, Krüppel-like factor 6 (KLF6), plays an important role in both the development and the progression of cancer. However, the effect of wild type KLF6 (wtKLF6) on the growth potential of renal carcinoma cells has not been examined. In the present study, we therefore introduced wtKLF6 gene into a prostatic carcinoma derived cell line, DU145, and a renal carcinoma derived cell line, OS-RC-2, and have established DU145-KLF6 and OS-RC-2-KLF6 cell lines, both of which stably over-express KLF6. Compared with vector-transfected cell lines (control cells), the wtKLF6-transfected cell lines showed the lower proliferation capacity (p < 0.01) and higher percentages of cells with apoptotic signals (p < 0.01). Moreover, the KLF6-overexpressed cell lines showed significant increases in the cell population at G0/G1 phase and significant decreases in the cell population at S and G2/M phases. There was no significant difference in the results of the cell cycle analysis between the two KLF6-overexpressed cell lines, DU145-KLF6 and OS-RC-2-KLF6. The cyclin-dependent kinase inhibitor p21 as a transcriptional target of the KLF6 gene was also studied. The expression levels of p21 mRNA and protein were up-regulated in both KLF6-overexpressed cell lines. These results indicate that the wtKLF6 gene effectively inhibited the growth of the prostatic carcinoma DU145 and renal carcinoma OS-RC-2 cell lines, probably through up-regulation of p21. Thus, KLF6 may represent a novel therapeutic target for inhibiting prostate and renal cancer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1349-3329
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
216
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-45
pubmed:meshHeading
pubmed-meshheading:18719336-Adenocarcinoma, pubmed-meshheading:18719336-Apoptosis, pubmed-meshheading:18719336-Carcinoma, Renal Cell, pubmed-meshheading:18719336-Cell Cycle, pubmed-meshheading:18719336-Cell Division, pubmed-meshheading:18719336-Cell Line, Tumor, pubmed-meshheading:18719336-Cyclin-Dependent Kinase Inhibitor p21, pubmed-meshheading:18719336-Gene Expression Regulation, Neoplastic, pubmed-meshheading:18719336-Humans, pubmed-meshheading:18719336-Kidney Neoplasms, pubmed-meshheading:18719336-Kruppel-Like Transcription Factors, pubmed-meshheading:18719336-Male, pubmed-meshheading:18719336-Neoplasm Proteins, pubmed-meshheading:18719336-Prostatic Neoplasms, pubmed-meshheading:18719336-Proto-Oncogene Proteins, pubmed-meshheading:18719336-RNA, Messenger, pubmed-meshheading:18719336-RNA, Neoplasm, pubmed-meshheading:18719336-Recombinant Fusion Proteins, pubmed-meshheading:18719336-Transcription, Genetic, pubmed-meshheading:18719336-Transfection
pubmed:year
2008
pubmed:articleTitle
Growth inhibitory effect of Krüppel-like factor 6 on human prostatic carcinoma and renal carcinoma cell lines.
pubmed:affiliation
Department of Urology, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't