Linked Life Data

18717965

Source:http://linkedlifedata.com/resource/pubmed/id/18717965

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-8-22
pubmed:databankReference
pubmed:abstractText
CMYA4 (cardiomyopathy-associated 4) gene plays an important role in thick filament assembly. In this study, we obtained the mRNA sequence including the full coding sequence and the partial 5' untranslated region of the porcine CMYA4 gene by using the rapid amplification of cDNA ends and reverse transcriptase polymerase chain reaction (RT-PCR) and the sequence was deposited in the GenBank nucleotide database (DQ_286571). The human (NM_173167) and mouse (NM_178680) homologues have a 91% and 87% identity with the porcine CMYA4 gene, respectively. The sequence contains an open reading frame encoding 930 amino acid residues, and the amino terminus of the predicted CMYA4 protein contains three tandem repeats belonging to the tetratricopeptide repeat family. Semi-quantitative RT-PCR results showed that the porcine CMYA4 gene is expressed exclusively in striated muscle tissue. An A558G single nucleotide polymorphism in the CMYA4 intron 15 detected as an MspI PCR-restriction fragment length polymorphism showed allele frequency differences among 225 unrelated pigs from six breeds. Association of the genotypes with growth and carcass traits showed that different genotypes of the CMYA4 gene were significantly associated with the backfat thickness of the area between sixth and seventh ribs (p < 0.05) and backfat thickness at the shoulder (p < 0.05).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0931-2668
pubmed:author
pubmed:issnType
Print
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
234-9
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Molecular characterization, expression and association analysis of the porcine CMYA4 gene with carcass traits.
pubmed:affiliation
Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't