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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2008-6-23
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pubmed:abstractText |
We sought to evaluate the effects of combined downregulation of CD134 and cytotoxic T lymphocyte-associated antigen 4 (CTLA4) on the autoimmune process of lupus. Concanavalin A (ConA)-induced proliferation, T helper cell cytokine secretion, and anti-double stranded DNA (dsDNA) antibody production were measured in cultures of splenic lymphocytes derived from lupus-prone BXSB mice. Splenocytes from six prednisone-treated and six untreated male lupus-prone BXSB mice, as well as from six syngeneically normal C57BL/6 male mice, were stimulated with ConA. BXSB splenocytes from untreated mice were exposed to anti-CD134L mAb, CTLA4 linked to the Fc portion of IgG1 (CTLA4Ig), or both. The magnitude of splenocyte proliferation and the levels of IFN-gamma, IL-6, and anti-dsDNA antibody were: (1) significantly higher in cultures of ConA-stimulated control and other cells than in unstimulated cells, (2) similar in cultures of normal and BXSB cells treated with anti-CD134 and CTLA4Ig or prednisone and (3) significantly reduced in cultures of ConA-stimulated and unstimulated cells treated with anti-CD134L and CTLA4Ig or prednisone compared with cells treated with CD134L or CTLA4Ig alone. Like corticosteroids, anti-CD134L mAb or CTLA4Ig can inhibit T- and B-cell activation by blocking the CD134-CD134L or CD28/CTLA4-B7 co-stimulatory pathway. The combined immune intervention described herein may prove useful for the treatment of autoimmune diseases such as systemic lupus erythematosus.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Antinuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoconjugates,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunosuppressive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/OX40 Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Prednisone,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, OX40,
http://linkedlifedata.com/resource/pubmed/chemical/abatacept
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1607-842X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
41
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
395-404
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:18568645-Animals,
pubmed-meshheading:18568645-Antibodies, Antinuclear,
pubmed-meshheading:18568645-Antibodies, Monoclonal,
pubmed-meshheading:18568645-B-Lymphocytes,
pubmed-meshheading:18568645-Cell Proliferation,
pubmed-meshheading:18568645-Cells, Cultured,
pubmed-meshheading:18568645-Concanavalin A,
pubmed-meshheading:18568645-Drug Therapy, Combination,
pubmed-meshheading:18568645-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:18568645-Flow Cytometry,
pubmed-meshheading:18568645-Gene Expression,
pubmed-meshheading:18568645-Immunoconjugates,
pubmed-meshheading:18568645-Immunoglobulin G,
pubmed-meshheading:18568645-Immunosuppressive Agents,
pubmed-meshheading:18568645-Interferon-gamma,
pubmed-meshheading:18568645-Interleukin-6,
pubmed-meshheading:18568645-Lupus Erythematosus, Systemic,
pubmed-meshheading:18568645-Male,
pubmed-meshheading:18568645-Mice,
pubmed-meshheading:18568645-OX40 Ligand,
pubmed-meshheading:18568645-Prednisone,
pubmed-meshheading:18568645-RNA, Messenger,
pubmed-meshheading:18568645-Receptors, OX40,
pubmed-meshheading:18568645-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18568645-Spleen,
pubmed-meshheading:18568645-Th1 Cells,
pubmed-meshheading:18568645-Th2 Cells
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pubmed:year |
2008
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pubmed:articleTitle |
Effect of anti-CD134L mAb and CTLA4Ig on ConA-induced proliferation, Th cytokine secretion, and anti-dsDNA antibody production in spleen cells from lupus-prone BXSB mice.
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pubmed:affiliation |
Department of Internal Medicine, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China. sumszyb@yahoo.com.cn
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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