Linked Life Data

18550297

Source:http://linkedlifedata.com/resource/pubmed/id/18550297

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2008-6-24
pubmed:abstractText
We report here a system for the functional expression of the Flp recombinase in several actinomycetes: Streptomyces coelicolor, S. lividans, and Saccharotrix espanaensis. We have constructed a synthetic gene encoding the Flp recombinase with a GC content of 60.6% optimized for expression in high-GC bacteria. Using the synthetic flp(a) gene, we have removed an apramycin resistance gene flanked by FRT sites from the chromosome of actinomycetes with an efficiency of 40%. Sequencing the region of chromosome showed that excision of the apramycin cassette by Flp recombinase was specific.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
419
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
43-7
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Marker removal from actinomycetes genome using Flp recombinase.
pubmed:affiliation
Albert-Ludwigs-University of Freiburg, Pharmazeutische Biologie und Biotechnologie, Stefan-Meier st. 19, Freiburg 79104, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't