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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1976-12-30
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pubmed:abstractText |
A double antibody radioimmunoassay technique was developed for quantification of apolipoprotein A-I, the major apoprotein of rat high density lipoprotein. Apo A-I was labeled with 125I by the chloramine-T method. 125I-labeled apo A-I had the same electrophoretic mobility as unlabeled apo A-I and more than 80% of the 125I was precipitated by rabbit anti apo A-I antibodies. The assay is sensitive at the level of 0.5-5 ng, and has intraassay and interassay coefficients of variation of 4.5 and 6.5% respectively. The specificity of the assay was established by competitive displacement of 125I-labeled apo A-I from its antibody by apo A-I and lipoproteins containing apo A-I, but not by rat albumin and other apoproteins. Immunoreactivity of high density lipoprotein and serum was only about 35% of that of their delipidated forms when Veronal buffer was used as a diluent. Inclusion of 5 mM sodium decyl sulfate in the incubation mixture brought out reactivity equivalent to that found after delipidation. Completeness of the reaction was verified by comparison with the amount of apo A-I in chromatographic fractions of the total apoprotein of high density lipoprotein. Content (weight %, mean values +/- S.D.) of immunoassayable apo A-I was: 62.3 +/- 5.9 in high density lipoprotein; 1.7 +/- 0.3 in low density lipoprotein; 0.09 +/- 0.03 in very low density lipoprotein and 25.0 +/- 5.0 in lymp chylomicrons. Concentration in whole serum was 51.4 +/- 8.9 mg/dl and 33.6 +/- 4.1 mg/dl for female and male rats, respectively (p less than 0.002), equivalent to the sex difference in concentration of high density lipoprotein. 95% of the apo A-I in serum was in high density lipoprotein, 5% in proteins of d greater than 1.21 g/ml and less than 1% in lipoproteins of d less than 1.063 g/ml.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
446
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
56-68
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:184835-Animals,
pubmed-meshheading:184835-Apolipoproteins,
pubmed-meshheading:184835-Binding Sites, Antibody,
pubmed-meshheading:184835-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:184835-Female,
pubmed-meshheading:184835-Lipoproteins, HDL,
pubmed-meshheading:184835-Male,
pubmed-meshheading:184835-Precipitin Tests,
pubmed-meshheading:184835-Radioimmunoassay,
pubmed-meshheading:184835-Rats,
pubmed-meshheading:184835-Sodium Dodecyl Sulfate
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pubmed:year |
1976
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pubmed:articleTitle |
Radioimmunoassay of apolipoprotein A-I of rat serum.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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