Linked Life Data

18434744

Source:http://linkedlifedata.com/resource/pubmed/id/18434744

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-7-16
pubmed:abstractText
The small (S) envelope protein is the major component of hepatitis B virus surface antigen (HBsAg). Some mutations in the S-HBsAg coding region may cause deficiency in the secretion of both viral and empty subviral particles (SVPs) and lead to accumulation of HBsAg inside the cells. In this study, we identified a unique amino acid substitution (L215Q) in the carboxyl-terminal end of S-HBsAg of an HBV genotype F isolate that provoked an inhibitory effect on secretion of SVPs. HBsAg levels were measured daily by enzyme-linked immunosorbent assay in the medium and cell extracts of HuH7 and CHO cells transiently transfected with plasmids containing wild-type or mutated S-HBsAg gene. Wild-type HBsAg was detectable in both medium and cell extracts of transfected cells. In contrast, extracellular levels of mutant HBsAg were not higher than cut-off values. By immunofluorescence with monoclonal antibody, it was shown that wild-type HBsAg was distributed throughout the cytoplasm, whereas mutant HBsAg was concentrated around the nucleus, suggesting retention in the endoplasmic reticulum. Amino acid substitutions that inhibit HBsAg secretion, such as that characterized in this study (L215Q), should have implications in HBV immunological diagnostics.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1423-0100
pubmed:author
pubmed:copyrightInfo
Copyright 2008 S. Karger AG, Basel.
pubmed:issnType
Electronic
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
81-6
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
A unique amino acid substitution, L215Q, in the hepatitis B virus small envelope protein of a genotype F isolate that inhibits secretion of hepatitis B virus subviral particles.
pubmed:affiliation
Laboratório de Virologia Molecular, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil. nmaraujo@ioc.fiocruz.br
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't