| pubmed-article:18395062 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18395062 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:18395062 | lifeskim:mentions | umls-concept:C0042036 | lld:lifeskim |
| pubmed-article:18395062 | lifeskim:mentions | umls-concept:C0872318 | lld:lifeskim |
| pubmed-article:18395062 | lifeskim:mentions | umls-concept:C2003903 | lld:lifeskim |
| pubmed-article:18395062 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:18395062 | pubmed:dateCreated | 2008-4-8 | lld:pubmed |
| pubmed-article:18395062 | pubmed:abstractText | 19-Nortestosterone (nandrolone) major metabolites in human urine are excreted as sulfoconjugated and glucuroconjugated forms. A sensitive and selective liquid chromatography/tandem mass spectrometry (LC/MS/MS) method in negative ESI mode was developed for direct quantification of 19-norandrosterone sulfate (19-NAS) and 19-noretiocholanolone sulfate (19-NES). For both sulfoconjugates, the [M-H](-) ion at m/z 355 and the fragment ion at m/z 97 were used as the precursor and product ions, respectively. The purification method involved a complete and rapid separation of sulfates and glucuronides in two extracts after loading the sample on a weak anion exchange solid phase extraction support (SPE Oasis WAX). Then, sulfates were separated by LC (Uptisphere ODB, 150 mm x 3.0 mm, 5 microm) and analyzed on a linear trap and a triple quadrupole mass spectrometer. The lower limit of detection (LLOD) and lowest limit of quantification (LLOQ) were of 100 pg mL(-1) and 1 ng mL(-1), respectively. Assay validation demonstrated good performances in terms of trueness (92.0-104.9%), repeatability (0.6-7.2%) and intermediate precision (1.3-10.8%) over the range of 1-2500 ng mL(-1). Finally, 19-NAS and 19-NES in urine samples collected after intake of 19-norandrostenedione (nandrolone precursor) were quantified. This assay may be easily implemented to separate glucuronide and sulfate steroids from urine specimens prior to quantification by LC/MS/MS. | lld:pubmed |
| pubmed-article:18395062 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18395062 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18395062 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18395062 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18395062 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18395062 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:18395062 | pubmed:issn | 1873-4324 | lld:pubmed |
| pubmed-article:18395062 | pubmed:author | pubmed-author:VeutheyJean-L... | lld:pubmed |
| pubmed-article:18395062 | pubmed:author | pubmed-author:SaugyMartialM | lld:pubmed |
| pubmed-article:18395062 | pubmed:author | pubmed-author:SaudanChristo... | lld:pubmed |
| pubmed-article:18395062 | pubmed:author | pubmed-author:RudazSergeS | lld:pubmed |
| pubmed-article:18395062 | pubmed:author | pubmed-author:StrahmEmmanue... | lld:pubmed |
| pubmed-article:18395062 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18395062 | pubmed:day | 21 | lld:pubmed |
| pubmed-article:18395062 | pubmed:volume | 613 | lld:pubmed |
| pubmed-article:18395062 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18395062 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18395062 | pubmed:pagination | 228-37 | lld:pubmed |
| pubmed-article:18395062 | pubmed:meshHeading | pubmed-meshheading:18395062... | lld:pubmed |
| pubmed-article:18395062 | pubmed:meshHeading | pubmed-meshheading:18395062... | lld:pubmed |
| pubmed-article:18395062 | pubmed:meshHeading | pubmed-meshheading:18395062... | lld:pubmed |
| pubmed-article:18395062 | pubmed:meshHeading | pubmed-meshheading:18395062... | lld:pubmed |
| pubmed-article:18395062 | pubmed:meshHeading | pubmed-meshheading:18395062... | lld:pubmed |
| pubmed-article:18395062 | pubmed:meshHeading | pubmed-meshheading:18395062... | lld:pubmed |
| pubmed-article:18395062 | pubmed:meshHeading | pubmed-meshheading:18395062... | lld:pubmed |
| pubmed-article:18395062 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18395062 | pubmed:articleTitle | Profiling of 19-norsteroid sulfoconjugates in human urine by liquid chromatography mass spectrometry. | lld:pubmed |
| pubmed-article:18395062 | pubmed:affiliation | Laboratoire Suisse d'Analyse du Dopage, Institut Universitaire de Médecine Légale, Chemin des Croisettes 22, 1066 Epalinges, Switzerland. | lld:pubmed |
| pubmed-article:18395062 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18395062 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:18395062 | pubmed:publicationType | Validation Studies | lld:pubmed |
