| pubmed-article:18364468 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18364468 | lifeskim:mentions | umls-concept:C0740009 | lld:lifeskim |
| pubmed-article:18364468 | lifeskim:mentions | umls-concept:C1136227 | lld:lifeskim |
| pubmed-article:18364468 | lifeskim:mentions | umls-concept:C0085304 | lld:lifeskim |
| pubmed-article:18364468 | lifeskim:mentions | umls-concept:C0596119 | lld:lifeskim |
| pubmed-article:18364468 | lifeskim:mentions | umls-concept:C0001473 | lld:lifeskim |
| pubmed-article:18364468 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:18364468 | lifeskim:mentions | umls-concept:C0332281 | lld:lifeskim |
| pubmed-article:18364468 | lifeskim:mentions | umls-concept:C0033727 | lld:lifeskim |
| pubmed-article:18364468 | lifeskim:mentions | umls-concept:C2828389 | lld:lifeskim |
| pubmed-article:18364468 | lifeskim:mentions | umls-concept:C1710425 | lld:lifeskim |
| pubmed-article:18364468 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:18364468 | pubmed:dateCreated | 2008-4-30 | lld:pubmed |
| pubmed-article:18364468 | pubmed:abstractText | Polarized growth in pollen tubes results from exocytosis at the tip and is associated with conspicuous polarization of Ca(2+), H(+), K(+), and Cl(-) -fluxes. Here, we show that cell polarity in Nicotiana tabacum pollen is associated with the exclusion of a novel pollen-specific H(+)-ATPase, Nt AHA, from the growing apex. Nt AHA colocalizes with extracellular H(+) effluxes, which revert to influxes where Nt AHA is absent. Fluorescence recovery after photobleaching analysis showed that Nt AHA moves toward the apex of growing pollen tubes, suggesting that the major mechanism of insertion is not through apical exocytosis. Nt AHA mRNA is also excluded from the tip, suggesting a mechanism of polarization acting at the level of translation. Localized applications of the cation ionophore gramicidin A had no effect where Nt AHA was present but acidified the cytosol and induced reorientation of the pollen tube where Nt AHA was absent. Transgenic pollen overexpressing Nt AHA-GFP developed abnormal callose plugs accompanied by abnormal H(+) flux profiles. Furthermore, there is no net flux of H(+) in defined patches of membrane where callose plugs are to be formed. Taken together, our results suggest that proton dynamics may underlie basic mechanisms of polarity and spatial regulation in growing pollen tubes. | lld:pubmed |
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| pubmed-article:18364468 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18364468 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18364468 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:18364468 | pubmed:status | MEDLINE | lld:pubmed |
