Linked Life Data

18339431

Source:http://linkedlifedata.com/resource/pubmed/id/18339431

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-4-8
pubmed:abstractText
Mycobacterium bovis is the causative agent of bovine tuberculosis (bTB). The proteins Ag85B, MPB64, and ESAT-6 are the major immunogenic antigens of M. bovis; these proteins play important roles in inducing immune responses that confer resistance against infections. In the present study, we used pcDNA3.1(+) as a vector and constructed various DNA vaccines with the genes encoding the three antigens mentioned above. This procedure involved the following steps: fusion of two genes (pcDNA-MPB64-Ag85B, pcMA), fusion of three genes (pcDNA-MPB64-Ag85B-ESAT-6, pcMAE), bivalent combinations (pcDNA-Ag85B+pcDNA-MPB64, pcA+M), and trivalent combinations (pcDNA-Ag85B+pcDNA-MPB64+pcDNA-ESAT-6, pcA+M+E). The immune response to the DNA vaccines was evaluated based on serum antibody titers, lymphocyte proliferation assay, and titers of the cytokines interferon-gamma (IFN-gamma) and interleukin-2 (IL-2). The protective efficacy following challenge with a virulent M. bovis strain, C68001, was evaluated based on survival rate, bacterial loads in lung tissue, and histopathologic changes. A significant 2-fold increase in serum antibody levels was observed in mice vaccinated with fusion DNA (two or three genes). Furthermore, the lymphocyte proliferation (SI) values and the levels of IFN-gamma and IL-2 were higher in mice vaccinated with fusion DNA (two or three genes) than in those immunized with polyvalent combination DNA vaccines (P<0.05). Additionally, the fusion DNA vaccines provided protection that was superior to that provided by the polyvalent combination DNA vaccines following challenge with M. bovis strain C68001. The protective efficacy of the fusion DNA vaccines in mice immunized three times was equivalent to the protective efficacy in mice immunized once with the Bacillus Calmette-Guerin (BCG) vaccine. This suggests that fusion DNA vaccine represent a promising approach for the prevention of bTB.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0165-2478
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
117
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
136-45
pubmed:meshHeading
pubmed-meshheading:18339431-Acyltransferases, pubmed-meshheading:18339431-Animals, pubmed-meshheading:18339431-Antigens, Bacterial, pubmed-meshheading:18339431-Bacterial Proteins, pubmed-meshheading:18339431-Cattle, pubmed-meshheading:18339431-Cloning, Molecular, pubmed-meshheading:18339431-DNA, Bacterial, pubmed-meshheading:18339431-Female, pubmed-meshheading:18339431-Immunity, Active, pubmed-meshheading:18339431-Immunization, pubmed-meshheading:18339431-Lung, pubmed-meshheading:18339431-Mice, pubmed-meshheading:18339431-Mice, Inbred BALB C, pubmed-meshheading:18339431-Mycobacterium bovis, pubmed-meshheading:18339431-Mycobacterium tuberculosis, pubmed-meshheading:18339431-Plasmids, pubmed-meshheading:18339431-Recombinant Fusion Proteins, pubmed-meshheading:18339431-Transfection, pubmed-meshheading:18339431-Tuberculosis, pubmed-meshheading:18339431-Tuberculosis, Bovine, pubmed-meshheading:18339431-Vaccines, DNA
pubmed:year
2008
pubmed:articleTitle
A novel DNA vaccine for protective immunity against virulent Mycobacterium bovis in mice.
pubmed:affiliation
Division of Bacterial Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 15000, PR China. siguo liu@yahoo.com.cn
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't