Linked Life Data

18328731

Source:http://linkedlifedata.com/resource/pubmed/id/18328731

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2008-4-18
pubmed:abstractText
Oral delivery of plasmid DNA (pDNA) is a desirable approach for fish immunization in intensive culture. However, its effectiveness is limited because of possible degradation of pDNA in the fish's digestive system. In this report, alginate microspheres loaded with pDNA coding for fish lymphocystis disease virus (LCDV) and green fluorescent protein were prepared with a modified oil containing water (W/O) emulsification method. Yield, loading percent and encapsulation efficiency of alginate microspheres were 90.5%, 1.8% and 92.7%, respectively. The alginate microspheres had diameters of less than 10 microm, and their shape was spherical. As compared to sodium alginate, a remarkable increase of DNA-phosphodiester and DNA-phosphomonoester bonds was observed for alginate microspheres loaded with pDNA by Fourier transform infrared (FTIR) spectroscopic analysis. Agarose gel electrophoresis showed a little supercoiled pDNA was transformed to open circular and linear pDNA during encapsulation. The cumulative release of pDNA in alginate microspheres was <or=10% in pH 2.0 acidic media, and it was less than 6.5% in pH 9.0 alkaline media in 12h. RT-PCR and immunofluorescence imaging indicated that pDNA expressed RNA and green fluorescent protein in tissues of fish 10-90 days after oral administration. An indirect enzyme-linked immunosorbent assay (ELISA) showed that sera were positive (OD >or=0.3) for anti-LCDV antibody from week 3 to week 16 for fish orally vaccinated with alginate microspheres loaded with pDNA, in comparison with fish orally vaccinated with naked pDNA. Our results display that alginate microspheres obtained by W/O emulsification are promising carriers for oral delivery of pDNA. This encapsulation technique has the potential for DNA vaccine delivery applications due to its ease of operation, low cost and significant immune effect.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1050-4648
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
592-9
pubmed:meshHeading
pubmed-meshheading:18328731-Administration, Oral, pubmed-meshheading:18328731-Alginates, pubmed-meshheading:18328731-Animals, pubmed-meshheading:18328731-Antibody Formation, pubmed-meshheading:18328731-DNA, Superhelical, pubmed-meshheading:18328731-DNA Virus Infections, pubmed-meshheading:18328731-Fish Diseases, pubmed-meshheading:18328731-Flounder, pubmed-meshheading:18328731-Glucuronic Acid, pubmed-meshheading:18328731-Green Fluorescent Proteins, pubmed-meshheading:18328731-Hexuronic Acids, pubmed-meshheading:18328731-Iridoviridae, pubmed-meshheading:18328731-Microspheres, pubmed-meshheading:18328731-Plasmids, pubmed-meshheading:18328731-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:18328731-Spectroscopy, Fourier Transform Infrared, pubmed-meshheading:18328731-Vaccines, DNA, pubmed-meshheading:18328731-Viral Vaccines
pubmed:year
2008
pubmed:articleTitle
Formation and oral administration of alginate microspheres loaded with pDNA coding for lymphocystis disease virus (LCDV) to Japanese flounder.
pubmed:affiliation
College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't