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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 3
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pubmed:dateCreated |
2008-3-5
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pubmed:abstractText |
Adipocytes play a central role in whole-body energy homoeostasis. Complex regulatory transcriptional networks control adipogensis, with ligand-dependent activation of PPARgamma (peroxisome proliferator-activated receptor gamma) being a decisive factor. Yet the identity of endogenous ligands promoting adipocyte differentiation has not been established. Here we present a critical evaluation of the role of LOXs (lipoxygenases) during adipocyte differentiation of 3T3-L1 cells. We show that adipocyte differentiation of 3T3-L1 preadipocytes is inhibited by the general LOX inhibitor NDGA (nordihydroguaiaretic acid) and the 12/15-LOX selective inhibitor baicalein. Baicalein-mediated inhibition of adipocyte differentiation was rescued by administration of rosiglitazone. Treatment with baicalein during the first 4 days of the differentiation process prevented adipocyte differentiation; supplementation with rosiglitazone during the same period was sufficient to rescue adipogenesis. Accordingly, we demonstrate that adipogenic conversion of 3T3-L1 cells requires PPARgamma ligands only during the first 4 days of the differentiation process. We show that the baicalein-sensitive synthesis of endogenous PPARgamma ligand(s) increases rapidly upon induction of differentiation and reaches a maximum on days 3-4 of the adipocyte differentiation programme. The conventional platelet- and leucocyte-type 12(S)-LOXs and the novel eLOX-3 (epidermis-type LOX-3) are expressed in white and brown adipose tissue, whereas only eLOX-3 is clearly expressed in 3T3-L1 cells. We suggest that endogenous PPARgamma ligand(s) promoting adipocyte differentiation are generated via a baicalein-sensitive pathway involving the novel eLOX-3.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/12-15-lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate 12-Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate 15-Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Flavanones,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Nordihydroguaiaretic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/PPAR gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazolidinediones,
http://linkedlifedata.com/resource/pubmed/chemical/baicalein,
http://linkedlifedata.com/resource/pubmed/chemical/rosiglitazone
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1470-8728
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pubmed:author |
pubmed-author:AmriEz-ZoubirEZ,
pubmed-author:BergeRolf KRK,
pubmed-author:FlecknerJanJ,
pubmed-author:FurstenbergerGerhardG,
pubmed-author:HallenborgPhilipP,
pubmed-author:JørgensenClausC,
pubmed-author:KriegPeterP,
pubmed-author:KristiansenKarstenK,
pubmed-author:MadsenLiseL,
pubmed-author:PetersenRasmus KRK,
pubmed-author:PridalLoneL,
pubmed-author:SørensenMorten BMB
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
375
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
539-49
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:18320708-3T3-L1 Cells,
pubmed-meshheading:18320708-Adipocytes,
pubmed-meshheading:18320708-Adipogenesis,
pubmed-meshheading:18320708-Adipose Tissue, Brown,
pubmed-meshheading:18320708-Adipose Tissue, White,
pubmed-meshheading:18320708-Animals,
pubmed-meshheading:18320708-Arachidonate 12-Lipoxygenase,
pubmed-meshheading:18320708-Arachidonate 15-Lipoxygenase,
pubmed-meshheading:18320708-Dose-Response Relationship, Drug,
pubmed-meshheading:18320708-Flavanones,
pubmed-meshheading:18320708-Ligands,
pubmed-meshheading:18320708-Lipoxygenase,
pubmed-meshheading:18320708-Lipoxygenase Inhibitors,
pubmed-meshheading:18320708-Mice,
pubmed-meshheading:18320708-Nordihydroguaiaretic Acid,
pubmed-meshheading:18320708-PPAR gamma,
pubmed-meshheading:18320708-Thiazolidinediones,
pubmed-meshheading:18320708-Time Factors,
pubmed-meshheading:18320708-Transcriptional Activation
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pubmed:year |
2003
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pubmed:articleTitle |
Adipocyte differentiation of 3T3-L1 preadipocytes is dependent on lipoxygenase activity during the initial stages of the differentiation process.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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