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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2008-2-25
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pubmed:abstractText |
Wnts are secreted glycoproteins that regulate important cellular processes including proliferation, differentiation, and cell fate. In the beta-catenin/canonical pathway, Wnt interacts with Fzd receptors to inhibit degradation of beta-catenin and promote its translocation into the nucleus where it regulates transcription of a number of genes. Dysregulation of this pathway has been attributed to a host of diseases including cancer. As a result, components of the beta-catenin/canonical pathway have been gaining recognition as promising targets for the discovery of novel therapeutic agents. Here, we show, using an ELISA-based protein-protein binding assay that purified Wnt7a binds to the extracellular cysteine-rich domain of Fzd5 in the nanomolar range. We have developed a novel split eGFP complementation assay to visually detect Wnt7a-Fzd5 interactions and subsequent pathway activation in cells. These biological tools could help lead to a better understanding of Wnt-Fzd interactions and the identification of new modulators of Wnt signaling.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-10318916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-10330181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-10580987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-11452312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-12042868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-12205098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-12438689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-12471263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-12717451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-12771126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-12857724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-14731402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-15142975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-15284440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-15454087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-15473860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-15849265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-16423993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-16491118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-1668888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-17018644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-17139285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-17297461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-6297757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-9161407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-9601644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-9671592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-9727977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-9767131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18230341-9867820
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1090-2104
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
4
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pubmed:volume |
368
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
285-91
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
Wnt7a interaction with Fzd5 and detection of signaling activation using a split eGFP.
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pubmed:affiliation |
Department of Integrative Biology and Pharmacology, University of Texas Health Science Center Houston, 6431 Fannin Street, Houston, TX 77030, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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