Linked Life Data

18076572

Source:http://linkedlifedata.com/resource/pubmed/id/18076572

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2007-12-13
pubmed:abstractText
DNA supercoiling factor (SCF) generates unconstrained negative supercoils of DNA in conjunction with eukaryotic topoisomerase II. In Drosophila melanogaster, SCF localizes to puffs on polytene chromosomes and is required for dosage compensation via hypertranscripton of genes on the male X chromosome. The present study investigated the role of SCF on autosomes. Although RNAi knockdown of scf results in male lethality, some escapers showed anterior homeotic transformation of the male sixth abdominal segment, similar to that arising from reduced expression of Abdominal-B (Abd-B). Heterozygotes for an scf mutant allele (scf(1)) displayed suppression of Pc mutation-dependent posterior transformation and enhancement of anterior transformation caused by trxG mutations. The level of Abd-B mRNA decreased in scf(1) embryos compared with wild-type. Tiling array experiments showed the presence of significant SCF signals in an Abd-B promoter region. Expression from the basal Abd-B promoter on a transgene was reduced in scf(1) embryos compared with wild-type. Collectively, these results demonstrate that SCF occupies the promoter region of Abd-B and activates expression for the proper formation of abdominal segments. Furthermore, preferential occupancy of SCF around transcription start sites of many active genes suggests a role for the factor in positive regulation of promoters.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1356-9597
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1347-55
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
DNA supercoiling factor positively regulates expression of the homeotic gene Abdominal-B in Drosophila melanogaster.
pubmed:affiliation
Department of Developmental Genetics, National Institute of Genetics, and Department of Genetics, SOKENDAI, Mishima, Shizuoka-ken 411-8540, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't