Linked Life Data

18004406

Source:http://linkedlifedata.com/resource/pubmed/id/18004406

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2008-3-4
pubmed:abstractText
Vaccination with recombinant viral vectors may be impeded by preexisting vector-specific immunity or by vector-specific immunity induced during the priming immunization. It is assumed that virus-neutralizing antibodies represent the principal effector mechanism of vector-specific immunity, while killing of infected cells by vector-specific cytotoxic T lymphocytes (CTLs) has also been suggested. Using recombinant Semliki Forest virus (rSFV) expressing E6E7 antigen from human papillomavirus, we demonstrate that secondary immune responses against E6E7 are neither affected by vector-specific antibodies nor by CTL-mediated killing of infected cells. Instead, the presence of the antigen during the prime immunization appeared to be the main determinant for the boosting efficacy. After priming with rSFVeE6,7, a homologous booster stimulated the primed E6E7-specific CTL response and induced long-lasting memory. Passively transferred SFV-neutralizing antibodies did not inhibit E6E7-specific CTL responses, although transgene expression was strongly reduced under these conditions. Conversely, in mice primed with irrelevant rSFV, induction of E6E7-specific CTLs was inhibited presumably due to vector-specific responses induced by the priming immunization. When during the priming with irrelevant rSFV, E7-protein was co-administered, the inhibitory effect of vector-specific immunity was abolished. These results suggest that, apart from vector-specific antibodies or killing of infected cells, T-cell competition may be involved in determining the efficacy of viral vector-based prime-boost immunization regimens.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1476-5462
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
393-403
pubmed:meshHeading
pubmed-meshheading:18004406-Animals, pubmed-meshheading:18004406-Antibodies, Monoclonal, pubmed-meshheading:18004406-DNA-Binding Proteins, pubmed-meshheading:18004406-Dose-Response Relationship, Immunologic, pubmed-meshheading:18004406-Female, pubmed-meshheading:18004406-Flow Cytometry, pubmed-meshheading:18004406-Gene Expression, pubmed-meshheading:18004406-Gene Therapy, pubmed-meshheading:18004406-Immunity, Cellular, pubmed-meshheading:18004406-Immunization, Secondary, pubmed-meshheading:18004406-Immunologic Memory, pubmed-meshheading:18004406-Luciferases, pubmed-meshheading:18004406-Lymphocyte Count, pubmed-meshheading:18004406-Mice, pubmed-meshheading:18004406-Mice, Inbred BALB C, pubmed-meshheading:18004406-Mice, Inbred C57BL, pubmed-meshheading:18004406-Oncogene Proteins, Viral, pubmed-meshheading:18004406-Semliki forest virus, pubmed-meshheading:18004406-T-Lymphocytes, pubmed-meshheading:18004406-T-Lymphocytes, Cytotoxic, pubmed-meshheading:18004406-Transgenes, pubmed-meshheading:18004406-Vaccines, Synthetic, pubmed-meshheading:18004406-Viral Vaccines, pubmed-meshheading:18004406-Virosomes
pubmed:year
2008
pubmed:articleTitle
Viral vector-based prime-boost immunization regimens: a possible involvement of T-cell competition.
pubmed:affiliation
Department of Medical Microbiology, Molecular Virology Section, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't