Source:http://linkedlifedata.com/resource/pubmed/id/17980607
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2008-2-1
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pubmed:abstractText |
Eupalinin A, a natural phytoalexin included in Eupatorium chinense L., exhibited a marked inhibitory effect on cell growth in HL60 cells. The morphological aspects of eupalinin A-treated cells evaluated by Hoechst 33342 nuclear staining indicated cell death, only a small part of which showed a typical apoptosis with nuclear fragmentation and condensation. To determine what type of cell death is caused by eupalinin A, we examined the contribution of caspases, Bcl-2 family proteins, MAP kinase, and PI3K/Akt, and mitochondrial membrane potential to this cell death. As a result, most part of the cell death was not associated with apoptosis because of caspase independence and no death factor released from mitochondria. Electron microscopic study indicated a characteristic finding of autophagy such as the formation of autophagosomes. Furthermore, the level of microctubule-associated-protein light chain 3 (LC3) II protein and monodancylcanaverin (MDC) incorporation were gradually increased with reduction of mitochondrial membrane potential by the accumulation of intracellular ROS after eupalinin A treatment. From these results, we can conclude that eupalinin A-induced cell death was mainly due to autophagy, which was initiated by increased ROS, resulting in the perturbation of mitochondrial membrane potential. Since the class III PI3K inhibitor such as 3-MA or LY294002 did not inhibit the eupalinin A-induced type II programmed cell death (PCD II), it was suggested that the PCD II was executed by Beclin-1 independent pathway of damage-induced mitochondrial autophagy (mitophagy).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1464-3391
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
721-31
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pubmed:meshHeading |
pubmed-meshheading:17980607-Apoptosis,
pubmed-meshheading:17980607-Autophagy,
pubmed-meshheading:17980607-Eupatorium,
pubmed-meshheading:17980607-HL-60 Cells,
pubmed-meshheading:17980607-Humans,
pubmed-meshheading:17980607-Lactones,
pubmed-meshheading:17980607-Molecular Structure,
pubmed-meshheading:17980607-Plants, Medicinal,
pubmed-meshheading:17980607-Sesquiterpenes,
pubmed-meshheading:17980607-Terpenes
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pubmed:year |
2008
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pubmed:articleTitle |
Eupalinin A isolated from Eupatorium chinense L. induces autophagocytosis in human leukemia HL60 cells.
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pubmed:affiliation |
Gifu International Institute of Biotechnology, 1-1 Naka-Fudogaoka, Kakamigahara, Gifu 504-0838, Japan. titoh@giib.or.jp
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pubmed:publicationType |
Journal Article
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