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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2007-10-30
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pubmed:abstractText |
X-chromosome inactivation (XCI) ensures the equality of X-chromosome dosages in male and female mammals by silencing one X in the female. To achieve the mutually exclusive designation of active X (Xa) and inactive X (Xi), the process necessitates that two Xs communicate in trans through homologous pairing. Pairing depends on a 15-kb region within the genes Tsix and Xite. Here, we dissect molecular requirements and find that pairing can be recapitulated by 1- to 2-kb subfragments of Tsix or Xite with little sequence similarity. However, a common denominator among them is the presence of the protein Ctcf, a chromatin insulator that we find to be essential for pairing. By contrast, the Ctcf-interacting partner, Yy1 (ref. 8), is not required. Pairing also depends on transcription. Transcriptional inhibition prevents new pair formation but does not perturb existing pairs. The kinetics suggest a pairing half-life of <1 h. We propose that pairing requires Ctcf binding and co-transcriptional activity of Tsix and Xite.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCCTC-binding factor,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Untranslated,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Tsix transcript, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/YY1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Yy1 protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1546-1718
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1390-6
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pubmed:meshHeading |
pubmed-meshheading:17952071-Animals,
pubmed-meshheading:17952071-Base Sequence,
pubmed-meshheading:17952071-Blastocyst,
pubmed-meshheading:17952071-Cell Differentiation,
pubmed-meshheading:17952071-Chromosomes, Mammalian,
pubmed-meshheading:17952071-DNA,
pubmed-meshheading:17952071-DNA-Binding Proteins,
pubmed-meshheading:17952071-Embryo, Mammalian,
pubmed-meshheading:17952071-Embryonic Stem Cells,
pubmed-meshheading:17952071-Female,
pubmed-meshheading:17952071-Gene Dosage,
pubmed-meshheading:17952071-Humans,
pubmed-meshheading:17952071-In Situ Hybridization, Fluorescence,
pubmed-meshheading:17952071-Male,
pubmed-meshheading:17952071-Mice,
pubmed-meshheading:17952071-Mice, Transgenic,
pubmed-meshheading:17952071-Molecular Sequence Data,
pubmed-meshheading:17952071-Protein Binding,
pubmed-meshheading:17952071-RNA, Small Interfering,
pubmed-meshheading:17952071-RNA, Untranslated,
pubmed-meshheading:17952071-Repressor Proteins,
pubmed-meshheading:17952071-Trans-Activators,
pubmed-meshheading:17952071-Transcription, Genetic,
pubmed-meshheading:17952071-X Chromosome,
pubmed-meshheading:17952071-X Chromosome Inactivation,
pubmed-meshheading:17952071-YY1 Transcription Factor,
pubmed-meshheading:17952071-Zinc Fingers
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pubmed:year |
2007
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pubmed:articleTitle |
Evidence that homologous X-chromosome pairing requires transcription and Ctcf protein.
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pubmed:affiliation |
Howard Hughes Medical Institute Department of Molecular Biology, Massachusetts General Hospital Department of Genetics, Harvard Medical School Boston, Massachusetts 02114, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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