Linked Life Data

17936911

Source:http://linkedlifedata.com/resource/pubmed/id/17936911

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-1-14
pubmed:abstractText
Direct measurement of neuropeptides in the hypothalamus is essential for neuroendocrine studies. However, the small quantities of peptides released at their neuroterminals and relatively large molecular sizes make these measurements difficult. We have evaluated microdialysis probes with two membrane materials (polycarbonate and polyarylethersulfone, both: molecular cut off 20,000 Da) in vitro, and adapted the method for in vivo hypothalamic sample collection in non-human primates. The results of in vitro experiments showed that the polyarylethersulfone membrane yielded a several fold higher recovery rate than the polycarbonate membrane. In in vivo experiments, a guide cannula with stylet was inserted into the medial basal hypothalamus through the permanently implanted cranial pedestal under light sedation. The stylet was replaced by a microdialysis probe and artificial CSF was infused. The results indicated that the neuropeptide luteinizing hormone-releasing hormone was readily measurable in dialysates collected at 10 min-intervals, and responded to neuroactive substances applied through the probe. The animals were fully conscious except for the initial hour of sampling. After the experiment the animal was returned to the home cage, and later similarly examined during several additional experiments. Therefore, the microdialysis method described here is a highly useful tool for neuroendocrine studies in non-human primates.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-10746653, http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-10804227, http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-11416013, http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-12488342, http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-12629203, http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-1373176, http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-1547745, http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-17112509, http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-2249640, http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-2661213, http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-3064879, http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-6889816, http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-7711193, http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-8264852, http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-8278400, http://linkedlifedata.com/resource/pubmed/commentcorrection/17936911-9579326
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
168
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
26-34
pubmed:dateRevised
2011-5-5
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Microdialysis methods for in vivo neuropeptide measurement in the stalk-median eminence in the Rhesus monkey.
pubmed:affiliation
Wisconsin National Primate Research Center, Northwestern University, Evanston, IL 60208-7180, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural