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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
1992-3-31
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pubmed:abstractText |
Plasmid DNA containing deoP1, one of the two major promoters of the deo operon, has been mutagenized using hydroxylamine, and promoter down-mutations and operator mutations were selected. The isolated mutants are all located within a 16 bp palindromic sequence containing the -10 region of deoP1. The results show that RNA polymerase and DeoR repressor compete for the same DNA target. The deoP1 promotor activity is dependent on a TG motif one base pair upstream of the -10 consensus sequence. The sequence of the deo operator site was further verified by use of a synthetic linker.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0950-382X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:geneSymbol |
deo,
deoP1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2371-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1791752-Base Sequence,
pubmed-meshheading:1791752-Cloning, Molecular,
pubmed-meshheading:1791752-DNA, Bacterial,
pubmed-meshheading:1791752-Escherichia coli,
pubmed-meshheading:1791752-Hydroxylamine,
pubmed-meshheading:1791752-Hydroxylamines,
pubmed-meshheading:1791752-Molecular Sequence Data,
pubmed-meshheading:1791752-Mutagenesis,
pubmed-meshheading:1791752-Oligodeoxyribonucleotides,
pubmed-meshheading:1791752-Operon,
pubmed-meshheading:1791752-Phenotype,
pubmed-meshheading:1791752-Plasmids,
pubmed-meshheading:1791752-Promoter Regions, Genetic,
pubmed-meshheading:1791752-Regulatory Sequences, Nucleic Acid
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pubmed:year |
1991
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pubmed:articleTitle |
deoP1 promoter and operator mutants in Escherichia coli: isolation and characterization.
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pubmed:affiliation |
Institute of Biological Chemistry B, University of Copenhagen, Denmark.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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