Linked Life Data

17822291

Source:http://linkedlifedata.com/resource/pubmed/id/17822291

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
2007-10-1
pubmed:abstractText
A novel, rapid, and economical method to isolate and purify natural sulforaphane from broccoli seeds is described. The procedure involves solvent extraction of autolyzed seed meal, followed by separation by solid phase extraction (SPE) and purification by preparative high-performance liquid chromatography (HPLC). The SPE method provides higher yield of sulforaphane from crude extracts compared to conventional liquid-liquid extraction. High purity and recovery of sulforaphane product can be obtained by preparative HPLC with a C 18 column and 30% methanol in water as the mobile phase. The purified compound was characterized by MS and (1)H and (13)C NMR. The techniques described here are useful tools in the preparative-scale isolation of sulforaphane in a fast, cost-effective, and waste-conscious manner.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-8561
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8047-53
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Separation and purification of sulforaphane from broccoli seeds by solid phase extraction and preparative high-performance liquid chromatography.
pubmed:affiliation
Key Laboratory of Bioprocess of Beijing, Beijing University of Chemical Technology, Beijing 100029, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't