Source:http://linkedlifedata.com/resource/pubmed/id/17714703
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
16
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| pubmed:dateCreated |
2007-9-21
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| pubmed:abstractText |
RhoA is a crucial regulator of stress fiber and focal adhesion formation through the activation of actin nucleation and polymerization. It also regulates the nuclear translocation of myocardin-related transcription factor-A and -B (MRTF-A/B, MAL or MKL 1/2), which are co-activators of serum response factor (SRF). In dominant-negative MRTF-A (DN-MRTF-A)-expressing NIH 3T3 cell lines, the expressions of several cytoskeletal/focal adhesion genes were down-regulated, and the formation of stress fiber and focal adhesion was severely diminished. MRTF-A/B-knockdown cells also exhibited such cytoskeletal defects. In reporter assays, both RhoA and MRTF-A enhanced promoter activities of these genes in a CArG-box-dependent manner, and DN-MRTF-A inhibited the RhoA-mediated activation of these promoters. In dominant-negative RhoA (RhoA-N19)-expressing NIH 3T3 cell lines, the nuclear translocation of MRTF-A/B was predominantly prevented, resulting in the reduced expression of cytoskeletal/focal adhesion proteins. Further, constitutive-active MRTF-A/B increased the expression of endogenous cytoskeletal/focal adhesion proteins, and thereby rescued the defective phenotype of stress fibers and focal adhesions in RhoA-N19 expressing cells. These results indicate that MRTF-A/B act as pivotal mediators of stress fiber and focal adhesion formation via the transcriptional regulation of a subset of cytoskeletal/focal adhesion genes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/MKL1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Response Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/myocardin-related transcription...,
http://linkedlifedata.com/resource/pubmed/chemical/rhoA GTP-Binding Protein
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0014-4827
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
313
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
3432-45
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:17714703-Actins,
pubmed-meshheading:17714703-Animals,
pubmed-meshheading:17714703-Cytoskeleton,
pubmed-meshheading:17714703-Focal Adhesions,
pubmed-meshheading:17714703-Gene Expression Regulation,
pubmed-meshheading:17714703-Genes, Dominant,
pubmed-meshheading:17714703-Humans,
pubmed-meshheading:17714703-Mice,
pubmed-meshheading:17714703-NIH 3T3 Cells,
pubmed-meshheading:17714703-Phenotype,
pubmed-meshheading:17714703-Promoter Regions, Genetic,
pubmed-meshheading:17714703-Protein Transport,
pubmed-meshheading:17714703-Serum Response Factor,
pubmed-meshheading:17714703-Stress Fibers,
pubmed-meshheading:17714703-Subcellular Fractions,
pubmed-meshheading:17714703-Trans-Activators,
pubmed-meshheading:17714703-Transcription, Genetic,
pubmed-meshheading:17714703-Transcription Factors,
pubmed-meshheading:17714703-rhoA GTP-Binding Protein
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| pubmed:year |
2007
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| pubmed:articleTitle |
Reorganization of the actin cytoskeleton via transcriptional regulation of cytoskeletal/focal adhesion genes by myocardin-related transcription factors (MRTFs/MAL/MKLs).
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| pubmed:affiliation |
Depertment of Neuroscience (D13), Osaka University Graduate School of Medicine, Yamadaoka 2-2, Suita, Osaka, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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