| pubmed-article:17696398 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17696398 | lifeskim:mentions | umls-concept:C0005532 | lld:lifeskim |
| pubmed-article:17696398 | lifeskim:mentions | umls-concept:C0015350 | lld:lifeskim |
| pubmed-article:17696398 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:17696398 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:17696398 | lifeskim:mentions | umls-concept:C0600484 | lld:lifeskim |
| pubmed-article:17696398 | lifeskim:mentions | umls-concept:C0813622 | lld:lifeskim |
| pubmed-article:17696398 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:17696398 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:17696398 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:17696398 | pubmed:dateCreated | 2007-9-10 | lld:pubmed |
| pubmed-article:17696398 | pubmed:abstractText | Hyaluronan (HA) derivatives containing thiol-reactive electrophilic esters were prepared to react with thiol-modified macromolecules to give cross-linker-free hydrogels. Specifically, HA was converted to two haloacetate derivatives, HA bromoacetate (HABA) and HA iodoacetate (HAIA). In cytotoxicity assays, these reactive macromolecules predictably induced cell death in a dose-dependent manner. Cross-linker-free synthetic extracellular matrix (sECM) hydrogels were prepared by thiol alkylation using HAIA and HABA as polyvalent electrophiles and thiol-modified HA (CMHA-S) with or without thiol-modified gelatin (Gtn-DTPH) as polyvalent nucleophiles. When primary human fibroblasts were seeded on the surface of the sECMs containing only the electrophilic HA haloacetate and nucleophilic CMHA-S components, no significant cytoadherence was observed. Cell attachment and viability was 17% (HABA) to 30% (HAIA) lower on HA haloacetate cross-linked hydrogels than on CMHA-S that had been oxidatively cross-linked via disulfide-bonds. In contrast, sECMs that included Gtn-DTPH allowed fibroblasts to attach, spread, and proliferate. Taken together, the HA haloacetates are attractive candidates for producing cross-linker-free sECM biomaterials that can function either as anti-adhesive barriers or as cytoadhesive sECMs for cell culture in pseudo-3-D. | lld:pubmed |
| pubmed-article:17696398 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17696398 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17696398 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17696398 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17696398 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17696398 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17696398 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17696398 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17696398 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17696398 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17696398 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17696398 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17696398 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:17696398 | pubmed:issn | 1525-7797 | lld:pubmed |
| pubmed-article:17696398 | pubmed:author | pubmed-author:PrestwichGlen... | lld:pubmed |
| pubmed-article:17696398 | pubmed:author | pubmed-author:SerbanMonica... | lld:pubmed |
| pubmed-article:17696398 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17696398 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:17696398 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17696398 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17696398 | pubmed:pagination | 2821-8 | lld:pubmed |
| pubmed-article:17696398 | pubmed:meshHeading | pubmed-meshheading:17696398... | lld:pubmed |
| pubmed-article:17696398 | pubmed:meshHeading | pubmed-meshheading:17696398... | lld:pubmed |
| pubmed-article:17696398 | pubmed:meshHeading | pubmed-meshheading:17696398... | lld:pubmed |
| pubmed-article:17696398 | pubmed:meshHeading | pubmed-meshheading:17696398... | lld:pubmed |
| pubmed-article:17696398 | pubmed:meshHeading | pubmed-meshheading:17696398... | lld:pubmed |
| pubmed-article:17696398 | pubmed:meshHeading | pubmed-meshheading:17696398... | lld:pubmed |
| pubmed-article:17696398 | pubmed:meshHeading | pubmed-meshheading:17696398... | lld:pubmed |
| pubmed-article:17696398 | pubmed:meshHeading | pubmed-meshheading:17696398... | lld:pubmed |
| pubmed-article:17696398 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17696398 | pubmed:articleTitle | Synthesis of hyaluronan haloacetates and biology of novel cross-linker-free synthetic extracellular matrix hydrogels. | lld:pubmed |
| pubmed-article:17696398 | pubmed:affiliation | Department of Medicinal Chemistry and Center for Therapeutic Biomaterials, The University of Utah, 419 Wakara Way, Suite 205, Salt Lake City, Utah 84108-1257, USA. | lld:pubmed |
| pubmed-article:17696398 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17696398 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17696398 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17696398 | lld:pubmed |
