| pubmed-article:17676063 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17676063 | lifeskim:mentions | umls-concept:C0035679 | lld:lifeskim |
| pubmed-article:17676063 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:17676063 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:17676063 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:17676063 | pubmed:dateCreated | 2007-9-7 | lld:pubmed |
| pubmed-article:17676063 | pubmed:abstractText | We imaged transcription in living cells using a locus-specific reporter system, which allowed precise, single-cell kinetic measurements of promoter binding, initiation and elongation. Photobleaching of fluorescent RNA polymerase II revealed several kinetically distinct populations of the enzyme interacting with a specific gene. Photobleaching and photoactivation of fluorescent MS2 proteins used to label nascent messenger RNAs provided sensitive elongation measurements. A mechanistic kinetic model that fits our data was validated using specific inhibitors. Polymerases elongated at 4.3 kilobases min(-1), much faster than previously documented, and entered a paused state for unexpectedly long times. Transcription onset was inefficient, with only 1% of polymerase-gene interactions leading to completion of an mRNA. Our systems approach, quantifying both polymerase and mRNA kinetics on a defined DNA template in vivo with high temporal resolution, opens new avenues for studying regulation of transcriptional processes in vivo. | lld:pubmed |
| pubmed-article:17676063 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17676063 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17676063 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17676063 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17676063 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17676063 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17676063 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17676063 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17676063 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:17676063 | pubmed:issn | 1545-9993 | lld:pubmed |
| pubmed-article:17676063 | pubmed:author | pubmed-author:SingerRobert... | lld:pubmed |
| pubmed-article:17676063 | pubmed:author | pubmed-author:Shav-TalYaron... | lld:pubmed |
| pubmed-article:17676063 | pubmed:author | pubmed-author:PhairRobert... | lld:pubmed |
| pubmed-article:17676063 | pubmed:author | pubmed-author:DarzacqXavier... | lld:pubmed |
| pubmed-article:17676063 | pubmed:author | pubmed-author:ShenoyShailes... | lld:pubmed |
| pubmed-article:17676063 | pubmed:author | pubmed-author:de... | lld:pubmed |
| pubmed-article:17676063 | pubmed:author | pubmed-author:BrodyYehudaY | lld:pubmed |
| pubmed-article:17676063 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17676063 | pubmed:volume | 14 | lld:pubmed |
| pubmed-article:17676063 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17676063 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17676063 | pubmed:pagination | 796-806 | lld:pubmed |
| pubmed-article:17676063 | pubmed:meshHeading | pubmed-meshheading:17676063... | lld:pubmed |
| pubmed-article:17676063 | pubmed:meshHeading | pubmed-meshheading:17676063... | lld:pubmed |
| pubmed-article:17676063 | pubmed:meshHeading | pubmed-meshheading:17676063... | lld:pubmed |
| pubmed-article:17676063 | pubmed:meshHeading | pubmed-meshheading:17676063... | lld:pubmed |
| pubmed-article:17676063 | pubmed:meshHeading | pubmed-meshheading:17676063... | lld:pubmed |
| pubmed-article:17676063 | pubmed:meshHeading | pubmed-meshheading:17676063... | lld:pubmed |
| pubmed-article:17676063 | pubmed:meshHeading | pubmed-meshheading:17676063... | lld:pubmed |
| pubmed-article:17676063 | pubmed:meshHeading | pubmed-meshheading:17676063... | lld:pubmed |
| pubmed-article:17676063 | pubmed:meshHeading | pubmed-meshheading:17676063... | lld:pubmed |
| pubmed-article:17676063 | pubmed:meshHeading | pubmed-meshheading:17676063... | lld:pubmed |
| pubmed-article:17676063 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17676063 | pubmed:articleTitle | In vivo dynamics of RNA polymerase II transcription. | lld:pubmed |
| pubmed-article:17676063 | pubmed:affiliation | Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA. | lld:pubmed |
| pubmed-article:17676063 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17676063 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:17676063 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17676063 | lld:pubmed |
