Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2007-6-21
pubmed:abstractText
1. The present study was designed to determine the effects of ginkgolide A, ginkgolide B and quercetin on CYP3A protein expression and enzyme activity in primary cultures of human hepatocytes. 2. Hepatocytes were pretreated with ginkgolide A, ginkgolide B and quercetin (at 1, 3, 10 and 30 micromol/L) for 48 h and then exposed to testosterone (250 micromol/L) for 30 min. Rifampin (10 micromol/L) and phenobarbital (2 mmol/L) were used as positive controls. The CYP3A activity was measured by the amount of 6beta-hydroxytestosterone in the culture medium and CYP3A protein in hepatocyte lysate was semiquantified by immunoblotting. 3. Compared with the vehicle control, ginkgolides A and B, at 30 micromol/L, significantly induced CYP3A protein expression (2.1- and 2-fold, respectively; both P < 0.01) and markedly induced CYP3A-mediated testosterone 6beta-hydroxylation (2.5-fold each; P < 0.05 for ginkgolide A; P > 0.05 for ginkgolide B). Quercetin had no apparent induction. 4. Ginkgolide A and ginkgolide B can induce CYP3A protein expression and enzyme activity in primary cultures of human hepatocytes at higher doses.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/CYP3A protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 CYP3A, http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System, http://linkedlifedata.com/resource/pubmed/chemical/Drugs, Chinese Herbal, http://linkedlifedata.com/resource/pubmed/chemical/Ginkgolides, http://linkedlifedata.com/resource/pubmed/chemical/Lactones, http://linkedlifedata.com/resource/pubmed/chemical/Phenobarbital, http://linkedlifedata.com/resource/pubmed/chemical/Quercetin, http://linkedlifedata.com/resource/pubmed/chemical/Rifampin, http://linkedlifedata.com/resource/pubmed/chemical/Testosterone, http://linkedlifedata.com/resource/pubmed/chemical/ginkgolide A, http://linkedlifedata.com/resource/pubmed/chemical/ginkgolide B
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0305-1870
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
632-5
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:17581220-Adult, pubmed-meshheading:17581220-Aged, pubmed-meshheading:17581220-Blotting, Western, pubmed-meshheading:17581220-Cells, Cultured, pubmed-meshheading:17581220-Cytochrome P-450 CYP3A, pubmed-meshheading:17581220-Cytochrome P-450 Enzyme System, pubmed-meshheading:17581220-Dose-Response Relationship, Drug, pubmed-meshheading:17581220-Drugs, Chinese Herbal, pubmed-meshheading:17581220-Enzyme Induction, pubmed-meshheading:17581220-Female, pubmed-meshheading:17581220-Ginkgolides, pubmed-meshheading:17581220-Hepatocytes, pubmed-meshheading:17581220-Herb-Drug Interactions, pubmed-meshheading:17581220-Humans, pubmed-meshheading:17581220-Hydroxylation, pubmed-meshheading:17581220-Lactones, pubmed-meshheading:17581220-Male, pubmed-meshheading:17581220-Middle Aged, pubmed-meshheading:17581220-Phenobarbital, pubmed-meshheading:17581220-Quercetin, pubmed-meshheading:17581220-Rifampin, pubmed-meshheading:17581220-Testosterone
pubmed:year
2007
pubmed:articleTitle
Induction of cyp3a in primary cultures of human hepatocytes by ginkgolides a and B.
pubmed:affiliation
Department of Pharmacology and Toxicology, Morehouse School of Medicine, Atlanta, GA 30310, USA. nhe@msm.edu
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural