rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2007-6-21
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pubmed:abstractText |
1. The present study was designed to determine the effects of ginkgolide A, ginkgolide B and quercetin on CYP3A protein expression and enzyme activity in primary cultures of human hepatocytes. 2. Hepatocytes were pretreated with ginkgolide A, ginkgolide B and quercetin (at 1, 3, 10 and 30 micromol/L) for 48 h and then exposed to testosterone (250 micromol/L) for 30 min. Rifampin (10 micromol/L) and phenobarbital (2 mmol/L) were used as positive controls. The CYP3A activity was measured by the amount of 6beta-hydroxytestosterone in the culture medium and CYP3A protein in hepatocyte lysate was semiquantified by immunoblotting. 3. Compared with the vehicle control, ginkgolides A and B, at 30 micromol/L, significantly induced CYP3A protein expression (2.1- and 2-fold, respectively; both P < 0.01) and markedly induced CYP3A-mediated testosterone 6beta-hydroxylation (2.5-fold each; P < 0.05 for ginkgolide A; P > 0.05 for ginkgolide B). Quercetin had no apparent induction. 4. Ginkgolide A and ginkgolide B can induce CYP3A protein expression and enzyme activity in primary cultures of human hepatocytes at higher doses.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CYP3A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 CYP3A,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Drugs, Chinese Herbal,
http://linkedlifedata.com/resource/pubmed/chemical/Ginkgolides,
http://linkedlifedata.com/resource/pubmed/chemical/Lactones,
http://linkedlifedata.com/resource/pubmed/chemical/Phenobarbital,
http://linkedlifedata.com/resource/pubmed/chemical/Quercetin,
http://linkedlifedata.com/resource/pubmed/chemical/Rifampin,
http://linkedlifedata.com/resource/pubmed/chemical/Testosterone,
http://linkedlifedata.com/resource/pubmed/chemical/ginkgolide A,
http://linkedlifedata.com/resource/pubmed/chemical/ginkgolide B
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0305-1870
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
632-5
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:17581220-Adult,
pubmed-meshheading:17581220-Aged,
pubmed-meshheading:17581220-Blotting, Western,
pubmed-meshheading:17581220-Cells, Cultured,
pubmed-meshheading:17581220-Cytochrome P-450 CYP3A,
pubmed-meshheading:17581220-Cytochrome P-450 Enzyme System,
pubmed-meshheading:17581220-Dose-Response Relationship, Drug,
pubmed-meshheading:17581220-Drugs, Chinese Herbal,
pubmed-meshheading:17581220-Enzyme Induction,
pubmed-meshheading:17581220-Female,
pubmed-meshheading:17581220-Ginkgolides,
pubmed-meshheading:17581220-Hepatocytes,
pubmed-meshheading:17581220-Herb-Drug Interactions,
pubmed-meshheading:17581220-Humans,
pubmed-meshheading:17581220-Hydroxylation,
pubmed-meshheading:17581220-Lactones,
pubmed-meshheading:17581220-Male,
pubmed-meshheading:17581220-Middle Aged,
pubmed-meshheading:17581220-Phenobarbital,
pubmed-meshheading:17581220-Quercetin,
pubmed-meshheading:17581220-Rifampin,
pubmed-meshheading:17581220-Testosterone
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pubmed:year |
2007
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pubmed:articleTitle |
Induction of cyp3a in primary cultures of human hepatocytes by ginkgolides a and B.
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pubmed:affiliation |
Department of Pharmacology and Toxicology, Morehouse School of Medicine, Atlanta, GA 30310, USA. nhe@msm.edu
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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