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rdf:type |
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lifeskim:mentions |
umls-concept:C0035126,
umls-concept:C0429403,
umls-concept:C0521447,
umls-concept:C1280500,
umls-concept:C1417708,
umls-concept:C1423613,
umls-concept:C1521761,
umls-concept:C1522564,
umls-concept:C1539081,
umls-concept:C1705525,
umls-concept:C1711351
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pubmed:issue |
2
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pubmed:dateCreated |
2007-7-27
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pubmed:abstractText |
Nuclear factor kappaB (NF-kappaB) is a ubiquitous transcription factor activated by various stimuli implicated in ischemia-reperfusion injury. However, the role of NF-kappaB in cardiac ischemia-reperfusion injury has not yet been well defined. Therefore, we investigated reperfusion damage in mice with targeted deletion of the NF-kappaB subunit p50. Electrophoretic mobility shift assays validated NF-kappaB activation in wild-type (WT) but not p50 knockout (KO) mice. KO and WT animals underwent 30 minutes of coronary artery ligation and 24 hours of reperfusion in vivo. Ischemia-reperfusion damage was significantly reduced in the p50 KO when compared with matching WT mice. Although adhesion molecules such as intercellular adhesion molecule were up-regulated in left ventricles of p50 KO animals, fewer neutrophils infiltrated the infarct area, suggesting leukocytes as a potential mediator of the protection observed in the p50 KO. This was confirmed in adoptive transfer experiments: whereas transplantation of KO bone marrow in KO animals sustained the protective effect on ischemia-reperfusion injury, transplantation of WT bone marrow in KO animals abolished it. Thus, deletion of the NF-kappaB subunit p50 reduces ischemia-reperfusion injury in vivo, associated with less neutrophil infiltration. Bone marrow transplantation experiments indicate that impaired NF-kappaB activation in p50 KO leukocytes attenuates cardiac damage.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-10229185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-10229233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-10430608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-10453042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-10475400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-10602461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-11083876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-11342480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-11726968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-12065235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-12920239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-12969313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-14512319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-14525778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-14574078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-14662734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-14962479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-14962493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-15194461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-15671153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-15853684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-15914117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-15921667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-16837548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-17060376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-17082652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-7603567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-7834752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-9003800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-9256281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-9788823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17556593-9798528
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0002-9440
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
171
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
507-12
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17556593-Animals,
pubmed-meshheading:17556593-Bone Marrow Cells,
pubmed-meshheading:17556593-Bone Marrow Transplantation,
pubmed-meshheading:17556593-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:17556593-Female,
pubmed-meshheading:17556593-Gene Expression,
pubmed-meshheading:17556593-Genotype,
pubmed-meshheading:17556593-Intercellular Adhesion Molecule-1,
pubmed-meshheading:17556593-Leukocytes,
pubmed-meshheading:17556593-Male,
pubmed-meshheading:17556593-Mice,
pubmed-meshheading:17556593-Mice, Inbred C57BL,
pubmed-meshheading:17556593-Mice, Knockout,
pubmed-meshheading:17556593-Myocardial Infarction,
pubmed-meshheading:17556593-Myocardial Reperfusion Injury,
pubmed-meshheading:17556593-Myocardium,
pubmed-meshheading:17556593-NF-kappa B p50 Subunit,
pubmed-meshheading:17556593-P-Selectin,
pubmed-meshheading:17556593-Tumor Necrosis Factor-alpha
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pubmed:year |
2007
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pubmed:articleTitle |
Tissue-specific effects of the nuclear factor kappaB subunit p50 on myocardial ischemia-reperfusion injury.
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pubmed:affiliation |
Medizinische Klinik und Poliklinik I, Herz-/Kreislaufzentrum, Universität Würzburg,Würzburg, Germany. frantz_s@medizin.uni-wuerzburg.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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