Linked Life Data

17487078

Source:http://linkedlifedata.com/resource/pubmed/id/17487078

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2007-5-9
pubmed:abstractText
Management of bone infection and resulting bony defects is one of the major issues in Orthopaedic surgery. Systemic antibiotic therapy alone does not usually eradicate bacteria because of poor penetration into bone. Therefore, local application of aminoglycoside antibiotics at the site of infection can provide high drug concentrations to eradicate the bacteria. Aminoglycosides have been shown to be toxic to certain cells in the ears and in the kidneys. Approximately 5-10% of the people who are treated with aminoglycosides experience some side effect, affecting their hearing, sense of balance, or kidneys. Little information exists in the literature addressing the effects of the aminoglycoside, Tobramycin, on osteoblast cells. MG63 osteoblast-like cell line, were treated with varying concentrations of Tobramycin (0, 10, 50, 100microM) over a period of 24, 48 and 72 hours, harvesting them after each time period and performing assays to test their metabolic function (Glutathione assay) and cell membrane viability (MDA). The results show that Tobramycin at concentrations greater than the minimum inhibitory concentration (MIC) caused marked reductions in cell number with increased membrane damage as early as 48 hours. Glutathione levels within the cells were increased in the 50uM and 100uM treatments as early as 24 hours indicating impaired metabolic function. These results may have implications for using Tobramycin as antibacterial prophylaxis in Orthopaedic surgery, as its toxic effects may interfere with the osteoblasts ability to form bone, and delay healing.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0067-8856
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
182-7
pubmed:dateRevised
2009-11-11
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
The effects of tobramycin on MG63 cell viability and function.
pubmed:affiliation
Howard University College of Medicine, Washington, DC, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't