Source:http://linkedlifedata.com/resource/pubmed/id/17411373
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2007-4-6
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pubmed:abstractText |
Leukemia inhibitor factor (LIF) has been shown to potently inhibit HIV-1 replication in vitro and in human organ explant cultures. Furthermore, LIF activates the Jak/Stat signaling pathway with which many viruses, including HIV-1, interfere. We used CXCR4 and the LIF signaling receptor (gp130)-expressing cMAGI cells transfected with CD4, CCR5, and HIV-LTR-beta-galactosidase as a model system to investigate the potential involvement of Stat proteins in the anti-HIV-1 effect of LIF. Pretreatment with recombinant human (rh)LIF resulted in a significantly reduced uptake of HIV-1(BaL) , HIV-1(LAI), and SIVmac251 viral particles without affecting uptake of murine leukemia retroviral particles. HIV-1(BaL), HIV-1(LAI), as well as rhLIF selectively induced phosphorylation of Stat 3 but not Stat 1 or Stat 5. However, treatment of cMAGI cells with rhLIF prior to HIV-1 infection downregulated the HIV-1-mediated Stat 3 phosphorylation. In addition, peripheral blood mononuclear cells (PBMCs) transfected with Stat 3 siRNA prior to HIV-1(LAI) or HIV-1(BaL) infection produced significantly less HIV-1 p24 antigen as compared to nontransfected HIV-1(LAI) and HIV-1(BaL)-infected PBMCs. Thus, the Jak/Stat signaling pathway is important for the HIV-1 replication life cycle and rhLIF excerts its anti-HIV-1 activity by disrupting this signaling cascade.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/HIV Core Protein p24,
http://linkedlifedata.com/resource/pubmed/chemical/LIF protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Leukemia Inhibitory Factor,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0889-2229
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
398-406
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pubmed:meshHeading |
pubmed-meshheading:17411373-Cell Line, Tumor,
pubmed-meshheading:17411373-HIV Core Protein p24,
pubmed-meshheading:17411373-HIV Infections,
pubmed-meshheading:17411373-HIV-1,
pubmed-meshheading:17411373-Humans,
pubmed-meshheading:17411373-Leukemia Inhibitory Factor,
pubmed-meshheading:17411373-Leukocytes, Mononuclear,
pubmed-meshheading:17411373-RNA, Small Interfering,
pubmed-meshheading:17411373-STAT3 Transcription Factor,
pubmed-meshheading:17411373-Signal Transduction,
pubmed-meshheading:17411373-Transfection,
pubmed-meshheading:17411373-Virus Replication
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pubmed:year |
2007
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pubmed:articleTitle |
Leukemia inhibitor factor (LIF) inhibits HIV-1 replication via restriction of stat 3 activation.
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pubmed:affiliation |
Center for Infectious Medicine, Department of Medicine, Karolinska University Hospital Huddinge, Karolinska Institutet, Stockholm, Sweden. Annelie.Tjernlund@ki.se
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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