Source:http://linkedlifedata.com/resource/pubmed/id/17393109
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2007-3-29
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| pubmed:abstractText |
In order to investigate the effect of antisense oligonucleotide (ASODN) of vascular endothelial growth factor C (VEGF-C) on lymphangiogenesis and angiogenesis of pancreatic cancer, antisense and scamble-sense oligonucleotide of VEGF-C were constructed, and the model of nude mice with orthotopically xenografted human pancreatic cancer cells (Panc-1) was established. Thirty nude mice were randomly divided into 3 groups: PBS control group (group A), scramble-sense control group (group B) and antisense group (group C). All nude mice were treated once every 2 days as 3 times per week, for 3 weeks (oligonucleotide 10 mg/kg every time). After treatments were completed, ELISA method was used to examine the concentration of VEGF-C in plasma and immunohistochemical method to examine microvessel density (MVD), lymphtic vessel density (LVD) of pancreatic cancer. The results showed that the expression of VEGF-C was inhibited significantly in group C. The concentrations were 237.5+/-41.5, 221.5+/-52.3 and 108.6+/-14.9 pg/mL in groups A, B and C respectively (P<0.01). LVD in groups A, B and C was 13.8+/-2.1, 12.4+/-1.9 and 4.2+/-1.6 respectively (P<0.01). MVD in groups A, B and C was 27.5+/-8.7, 25.9+/-4.2 and 19.4+/-5.6 respectively with no significant difference among the groups (P>0.05). It was suggested that VEGF-C ASODN decreased the expression levels of VEGF-C in nude mice with orthotopically xenografted human pancreatic cancer, and it could inhibit lymphangiogenesis, but had no significant effect on angiogenesis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Factor VIII,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor C,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor...
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1672-0733
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
27
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
51-3
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:17393109-Animals,
pubmed-meshheading:17393109-Cell Line, Tumor,
pubmed-meshheading:17393109-Disease Models, Animal,
pubmed-meshheading:17393109-Drug Administration Schedule,
pubmed-meshheading:17393109-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:17393109-Factor VIII,
pubmed-meshheading:17393109-Gene Therapy,
pubmed-meshheading:17393109-Humans,
pubmed-meshheading:17393109-Immunohistochemistry,
pubmed-meshheading:17393109-Lymphangiogenesis,
pubmed-meshheading:17393109-Mice,
pubmed-meshheading:17393109-Microcirculation,
pubmed-meshheading:17393109-Neovascularization, Pathologic,
pubmed-meshheading:17393109-Oligonucleotides, Antisense,
pubmed-meshheading:17393109-Pancreatic Neoplasms,
pubmed-meshheading:17393109-Random Allocation,
pubmed-meshheading:17393109-Vascular Endothelial Growth Factor C,
pubmed-meshheading:17393109-Vascular Endothelial Growth Factor Receptor-3,
pubmed-meshheading:17393109-Xenograft Model Antitumor Assays
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| pubmed:year |
2007
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| pubmed:articleTitle |
Effect of antisense oligodeoxynucleotide of vascular endothelial growth factor C on lymphangiogenesis and angiogenesis of pancreatic cancer.
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| pubmed:affiliation |
Department of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. tj918@163.com
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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